An investigation on tolerance and biosorption potential of Aspergillus awamori ZU JQ 965830.1 TO Cd(II)
An investigation on tolerance and biosorption potential of Aspergillus awamori ZU JQ 965830.1 TO Cd(II)
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DOI：
10.1007/s13213-014-0838-7
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发表时间：
2015-03
影响因子：
3
通讯作者：
M. El-Sayed
中科院分区：
文献类型：
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作者：
M. El-Sayed
泡盛曲霉对Cd（Ⅱ）的最低抑菌浓度（MIC）为700 mg/l。在400毫克/升浓度的镉（II）的生物量生产的完全抑制。通过扫描电镜观察发现，Cd（II）胁迫下的分生孢子梗和分生孢子发生了明显的变形. Cd（II）的定量通过EDX微量分析进行。透射电子显微镜检查（TEM）证实了细胞外吸附，细胞内渗透通过细胞壁和空泡化的参与。Cd（II）胁迫引起多酚氧化酶（PPO）、谷胱甘肽还原酶（GR）和过氧化物酶（POD）活性以及总抗氧化剂、可溶性蛋白和巯基含量的显著变化。高效液相色谱分析（HPLC）表明，镉（II）胁迫刺激草酸的生产。当pH为5.0，初始金属离子浓度为500 mg/L，生物量为1 g/L时，Cd（II）的吸收能力最大。最大镉（II）的吸收能力后6小时达到活生物量和死亡生物量后2小时。傅里叶变换红外光谱（FTIR）的结果给出了指示之间的螯合氧，氮，磷，特别是含硫的生物质配体与金属离子。X射线衍射分析（XRD）显示活的和死的生物质中存在CdSO 4·H2O。EDX证实了硫，氧和镉（II）的细胞壁上的发生。
The minimum inhibitory concentration (MIC) value ofAspergillus awamorifor Cd(II) was 700 mg/l. A complete inhibition of biomass production was observed at 400 mg/l concentration of Cd(II). A significant deformation in Cd(II)-stressed conidiophores and conidia was observed by Scanning electron microscopy (SEM) investigation. Quantification of Cd(II) was performed by EDX microanalysis. Transmission electron microscopy investigation (TEM) confirmed the involvement of extracellular adsorption, intracellular penetration through the cell wall and vacuolation. Cadmium(II) stress induced noticeable changes in the activities of polyphenol oxidase (PPO), glutathione reductase (GR) and peroxidase (POD), and in the concentrations of total antioxidants, soluble protein and thiols. High performance liquid chromatography analysis (HPLC) revealed that Cd(II) stress stimulated the production of oxalic acid. Maximum Cd(II) uptake capacity was achieved at pH 5.0, initial metal ion concentration 500 mg/l and biomass dosage 1 g/l. Maximum Cd(II) uptake capacity was reached after 6 h for live biomass and after 2 h for dead biomass. Fourier transform infrared spectroscopy (FTIR) results gave an indication on chelation between oxygen-, nitrogen-, phosphorus- and especially sulphur-containing ligands of biomass with metal ions. X-ray diffraction analysis (XRD) revealed the presence of CdSO4.H2O by live and dead biomass. EDX confirmed the occurrence of sulphur, oxygen and Cd(II) on the cell wall.