Genotypic characterization of human and environmental isolates of Salmonella choleraesuis subspecies choleraesuis serovar Infantis by pulsed-field gel electrophoresis
Genotypic characterization of human and environmental isolates of Salmonella choleraesuis subspecies choleraesuis serovar Infantis by pulsed-field gel electrophoresis
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DOI：
10.1111/j.1348-0421.1999.tb02406.x
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发表时间：
1999-01-01
影响因子：
2.6
通讯作者：
Otsuki, K
中科院分区：
文献类型：
--
作者：
Murakami, K;Horikawa, K;Otsuki, K
为确定霍乱沙门氏菌亚种婴儿型霍乱沙门氏菌的遗传多样性程度以及环境分离株与人类分离株是否相似或相同，采用脉冲场凝胶电泳法对110株来自人类、肉鸡样品、产蛋设施、河水、污水和鸡肉的分离株进行流行病学分析。虽然分离株显示35种不同的脉冲场谱，但没有一种具有人类分离株的基因型。110株分离株中有43株（39%）具有相同的脉冲场谱。这些结果表明，在日本西部广泛传播的S，血清型婴儿的克隆系相对较少，而包括此次暴发的菌株在内的多个克隆系则存在。
To determine the extent of genetic diversity of Salmonella choleraesuis subspecies choleraesuis serovar Infantis and whether environmental isolates were similar or identical to human isolates, a total of 110 isolates from humans, broiler samples, egg production facilities, riverwater, sewage, and chicken meat were analyzed epidemiologically by pulsed-field gel electrophoresis. While the isolates showed 35 distinct pulsed-field profiles, none had the genotype of the human isolates. One pulsed-field profile was shared by 43 (39%) of the 110 isolates. These results indicate that relatively fewer clonal lines of S, serovar Infantis had spread widely while multiple clonal lines, including the strain involved in the outbreak, exist in Western Japan.