Lipidomic investigations for the characterization of circulating serum lipids in multiple sclerosis
Lipidomic investigations for the characterization of circulating serum lipids in multiple sclerosis
复制标题
DOI：
10.1016/j.jprot.2011.06.023
复制
发表时间：
2011-11-18
影响因子：
3.3
通讯作者：
Urbani, Andrea
中科院分区：
文献类型：
--
作者：
Del Boccio, Piero;Pieragostino, Damiana;Urbani, Andrea
多发性硬化症 (MS) 是一种影响年轻人的神经退行性自身免疫性脱髓鞘疾病。病因学仍然是一个谜，并且由于缺乏明确的分子标志物而影响了诊断。自身免疫反应仍然是正在研究的主要课题，最近的研究表明脑部炎症还存在其他非蛋白质介质，例如脂质。我们采用基于 LC-MS 的脂质组学方法来突出 MS 中的血清脂质分析。该方法经过优化并应用于多发性硬化症患者与健康对照 (HC) 和其他神经系统疾病 (OND) 患者的初步临床横断面调查。通过精确质量和 MS/MS 实验突出显示并初步鉴定了十种重要的代谢物。我们最相关的数据显示溶血甘油磷脂酰胆碱 (lysoPC) 和甘油磷脂酰胆碱 (PC) 种类的水平发生变化。总 lysoPC/PC 比率在病理组（MS、OND）中显示显着下降，此外，MS 受试者的该比率相对于 OND 也有相关下降。这些发现表明，多发性硬化症中磷脂代谢可能发生改变，可以在血清中进行评估。其中一些特征是独特的，可能被认为是 MS 特有的。我们的脂质组学数据首次显示血清中 LysoPC/PC 比率与 MS 之间关系的证据。 (C) 2011 Elsevier B.V. 保留所有权利。
Multiple Sclerosis (MS) is a neurodegenerative autoimmune demyelinating disease affecting young adults. The aetiology still remains a mystery and diagnosis is impaired by the lack of defined molecular markers. Autoimmune response remains the main topic under investigation and recent studies suggest additional non-proteic mediators of brain inflammation such as lipids. We carried out an LC-MS based lipidomics approach to highlight serum lipids profiling in MS. Method was optimised and applied in a preliminary clinical cross-sectional investigation of MS patients vs Healthy Controls (HC) and patients with Other Neurological Diseases (OND). Ten significant metabolites were highlighted and tentatively identified by accurate mass and MS/MS experiments. Our most relevant data show altered level of lyso-glycerophosphatidylcholine (lysoPC) and glycerophosphatidylcholine (PC) species. Total lysoPC/PC ratio showed significant decrease in pathological groups (MS, OND) and, in addition, MS subjects had a relevant decrease of this ratio also in respect to OND. These findings suggest that there may be an altered phospholipid metabolism in MS that can be evaluated in serum. Some of these features are distinctive and may be considered specific for MS. Our lipidomics data show, for the first time, evidence in serum of a relationship between LysoPC/PC ratio and MS. (C) 2011 Elsevier B.V. All rights reserved.