Induction of endothelin secretion by angiotensin II: effects on growth and synthetic activity of vascular smooth muscle cells.
Induction of endothelin secretion by angiotensin II: effects on growth and synthetic activity of vascular smooth muscle cells.
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DOI：
10.1097/00005344-199100177-00025
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发表时间：
1991
影响因子：
3
通讯作者：
T. Scott‐Burden;T. Resink;A. Hahn;P. Vanhoutte
中科院分区：
文献类型：
--
作者：
T. Scott‐Burden;T. Resink;A. Hahn;P. Vanhoutte
血管紧张素II诱导培养的大鼠血管平滑肌细胞合成和分泌内皮素。以前的研究表明，血管紧张素II也激活细胞外基质相关的糖缀合物（糖肽和蛋白聚糖）的培养平滑肌细胞的合成。此外，在含有去丝裂原的血浆衍生血清（1.0%）的培养基中，血管紧张素II刺激大鼠平滑肌细胞的生长。因此，内皮素-1（ET-1）对培养的大鼠平滑肌细胞生长和基质形成的影响进行了研究，以评估其对血管紧张素II刺激活性的贡献。在平滑肌细胞的静止培养中，即使在最大剂量（10（-7）M）下，ET-1对[3 H]胸苷掺入DNA的刺激作用也不明显。在相同的条件下，合成的细胞外基质糖缀合物材料不增强ET-1，不像血管紧张素II。在培养基中含有1.0%血浆来源的血清，ET-1是不能刺激增殖或掺入[3 H]胸苷到DNA中，而血管紧张素II刺激这两种活动。然而，ET-1诱导生长因子和血小板反应蛋白基因在静止平滑肌培养物中的表达。这些数据表明，血管紧张素II对平滑肌细胞的生长刺激不会由于刺激的ET-1产生而发生。
Angiotensin II induces the synthesis and secretion of endothelin by cultured rat vascular smooth muscle cells. Previous studies demonstrate that angiotensin II also activates the synthesis of extracellular matrix-associated glycoconjugates (glycopeptides and proteoglycans) by cultured smooth muscle cells. Furthermore, in culture medium containing mitogen-depleted, plasma-derived serum (1.0%), angiotensin II stimulates the growth of rat smooth muscle cells. Therefore, the influence of endothelin-1 (ET-1) on the growth and matrix elaboration by cultured rat smooth muscle cells was studied to assess its contribution to the stimulatory activity of angiotensin II. In quiescent cultures of smooth muscle cells, no stimulation of the incorporation of [3H]thymidine into DNA by ET-1 was apparent, even at maximal doses (10(-7) M). Under the same conditions, the synthesis of extracellular matrix glycoconjugate material was not enhanced by ET-1, unlike angiotensin II. In cultures maintained in medium containing 1.0% plasma-derived serum, ET-1 was incapable of stimulating either proliferation or incorporation of [3H]thymidine into DNA whereas angiotensin II stimulated both activities. ET-1, however, did induce the expression of growth factor and thrombospondin genes in quiescent smooth muscle cultures. The data suggest that growth stimulation of smooth muscle cells by angiotensin II does not occur as a consequence of stimulated ET-1 production.