Social stability attenuates the stress in the modified multiple platform method for paradoxical sleep deprivation in the rat
Social stability attenuates the stress in the modified multiple platform method for paradoxical sleep deprivation in the rat
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DOI：
10.1016/s0031-9384(99)00181-x
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发表时间：
2000-01-01
影响因子：
2.9
通讯作者：
Tufik, S
中科院分区：
文献类型：
--
作者：
Suchecki, D;Tufik, S
诱导反常睡眠（PS）剥夺的工具方法是有压力的。改良的多平台方法 (MMPM) 将动物与新的群体一起放置在水箱内，可增强 ACTH 和皮质酮 (CORT) 反应。我们假设这种增加的反应可能归因于社会不稳定。此外，我们还测试了一种新的环境控制装置，即放置在储罐地板上的网格（GR）。将动物作为社会不稳定组（UG-来自多个笼子）或稳定组（SG-来自一个笼子）提交给 MMPM 4 天，放置在狭窄的平台上或网格上。所有 UG 动物的 ACTH 水平均高于 SG 动物，包括家笼对照。仅在稳定组中，实验动物的 CORT 水平高于对照组。 UG 动物的肾上腺比 SG 动物更重。只有放在网格上的 SG 动物的肾上腺与笼子对照相似。 SG 大鼠的体重减轻少于 UG 动物。虽然 UG 动物与家笼对照动物吃的食物量相同，但 SG 动物比对照动物和 UG 动物吃得更多。这些结果表明 MMPM 的压力可以在稳定的群体中减弱。只要考虑与应力相关的变量，在罐底引入网格可以提供充分的环境控制。 (C) 2000 Elsevier Science Inc. 保留所有权利。
The instrumental methods to induce paradoxical sleep (PS) deprivation are stressful. The modified multiple platform method (MMPM), in which animals are placed with new cohorts inside the water tanks, results in augmented ACTH and corticosterone (CORT) responses. We hypothesised that this increased response could be attributed to social instability. In addition, we tested a new environmental control, a grid (GR) placed on the tank floor. Animals were submitted to the MMPM for 4 days as socially unstable (UG-coming from several cages) or stable groups (SG-coming from one cage), placed either on narrow platforms or on the grid. All UG animals presented higher ACTH levels than their SG counterparts, including home-cage controls. CORT levels of manipulated animals were higher than controls only in the stable group. UG animals presented heavier adrenals than their SG counterparts. Only adrenals from SG animals placed on the grid were similar to cage controls. SG rats lost less weight than UG animals. While UG animals ate the same amount of chow as home-cage controls, SG animals ate more than control and UG animals. These results suggest that the stress of the MMPM can be attenuated in stable groups. The introduction of a grid on the tank floor may serve an adequate environmental control as far as stress related variables are considered. (C) 2000 Elsevier Science Inc. All rights reserved.