Primary airway epithelial cell culture from lung transplant recipients
Primary airway epithelial cell culture from lung transplant recipients
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DOI：
10.1183/09031936.05.00141404
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发表时间：
2005-12-01
影响因子：
24.3
通讯作者：
Corris, PA
中科院分区：
文献类型：
--
作者：
Forrest, IA;Murphy, DM;Corris, PA
肺移植的长期存活受到闭塞性细支气管炎的发展的限制，闭塞性细支气管炎是一种以炎症、上皮损伤、纤维增生和细支气管闭塞导致气流阻塞为特征的病症。为探讨支气管上皮细胞在闭塞性细支气管炎发病中的作用，本研究从同种异体肺移植物中分离支气管上皮细胞，建立支气管上皮细胞原代培养体系。将细胞接种在胶原包被的平板上，在支气管上皮生长培养基中生长至汇合。PBEC从12/33（39%）刷拭生长至汇合。未能达到融合是由于早期先天性感染。细菌通常从支气管肺泡灌洗液和培养基中分离出来，但仅在培养基中发现了一个单独的种群。尽管早期患者源性感染的发生率很高，但肺移植受者支气管上皮细胞的原代培养是可行的。只有通过支气管刷检才能发现的同种异体移植物的潜伏性感染本身可能是上皮损伤的持续刺激。这项技术有助于将来研究闭塞性细支气管炎发病机制中气道上皮反应的机制。
Long-term survival in lung transplantation is limited by the development of obliterative bronchiolitis, a condition characterised by inflammation, epithelial injury, fibroproliferation and obliteration of bronchioles leading to airflow obstruction. To investigate the role of the bronchial epithelium in the pathogenesis of obliterative bronchiolitis the current study aimed to establish primary bronchial epithelial cell cultures (PBEC) from lung allografts.Four to six bronchial brushings were obtained from sub-segmental bronchi of lung allografts. Cells were seeded onto collagen-coated plates and grown to confluence in bronchial epithelial growth medium.Bronchial brushings (n=33) were obtained from 27 patients. PBECs were grown to confluence from 12 out of 33 (39%) brushings. Failure to reach confluence was due to early innate infection. Bacteria were usually isolated from both bronchoalveolar lavage and culture media, but a separate population was identified in culture media only.Primary culture of bronchial epithelial cells from lung transplant recipients is feasible, despite a high rate of early, patient-derived infection. Latent infection of the allograft, identified only by bronchial brushings, may itself be a persistent stimulus for epithelial injury. This technique facilitates future mechanistic studies of airway epithelial responses in the pathogenesis of obliterative bronchiolitis.