MOLECULAR ANALYSIS AND CHROMOSOMAL MAPPING OF AMPLIFIED GENES ISOLATED FROM A TRANSFORMED MOUSE 3T3-CELL LINE
MOLECULAR ANALYSIS AND CHROMOSOMAL MAPPING OF AMPLIFIED GENES ISOLATED FROM A TRANSFORMED MOUSE 3T3-CELL LINE
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DOI：
10.1007/bf01535205
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发表时间：
1987-05-01
期刊：
影响因子：
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通讯作者：
GEORGE, DL
中科院分区：
文献类型：
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作者：
CAHILLYSNYDER, L;YANGFENG, T;GEORGE, DL
我们正在探索小鼠3T3细胞自发转化衍生物中与双分钟（DMs）相关的扩增DNA序列的起源和功能。为了实现这一目标，我们利用来自这些细胞的RNA构建了cDNA文库，并分离了cDNA克隆，这些克隆代表了在这些3T3-DM细胞中扩增和过表达的序列。根据Northern- blot和Southern-blot分析的结果，我们得出结论，这些cdna代表两个不同的基因，我们将其命名为mdm-1和mdm-2。利用一组中国仓鼠-小鼠体细胞杂交体的DNA以及基因定位研究的原位杂交方案，我们发现这些与dm相关的扩增DNA序列来自小鼠10号染色体C1-C3区。与mdm-1和mdm-2同源的序列存在于包括人类在内的几个物种的基因组中。
We are exploring the origin and function of amplified DNA sequences associated with double minutes (DMs) in a spontaneously transformed derivative of mouse 3T3 cells. Toward that goal, we have constructed a cDNA library using RNA from these cells and have isolated cDNA clones representing sequences that are amplified and overexpressed in these 3T3-DM cells. From results of Northern- and Southern-blot analyses, we conclude that these cDNAs represent two distinct genes, which we have designated mdm-1 and mdm-2. Using DNAs from a panel of Chinese hamster-mouse somatic cell hybrids together with in situ hybridization protocols for gene mapping studies, we have found that these DM-associated, amplified DNA sequences originate from mouse chromosome 10, region C1-C3. Sequences homologous to mdm-1 and mdm-2 are present in the genomes of several species examined, including that of man.