Regenerative Potential of Human Schneiderian Membrane: Progenitor Cells and Epithelial-Mesenchymal Transition
Regenerative Potential of Human Schneiderian Membrane: Progenitor Cells and Epithelial-Mesenchymal Transition
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DOI：
10.1002/ar.23276
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发表时间：
2015-12-01
影响因子：
2
通讯作者：
Rusu, M. C.
中科院分区：
文献类型：
--
作者：
Derjac-Arama, A. I.;Sarafoleanu, C.;Rusu, M. C.
在从非人类物种到人类受试者的研究中，对施奈德膜（SM）的先天成骨潜力进行了逐步评估。它与骨内膜放置和骨整合相关。表达巢蛋白的成骨祖细胞据称参与骨形成和重塑。巢蛋白表型没有评估以前在人类SM。因此，我们的目标是填补文献中的这一空白。在手术过程中从8名成人患者中获得成人SM的活检样本，这些患者因非恶性病变而手术。石蜡包埋组织样本的免疫组织化学使用抗巢蛋白、CD 45、CD 146、细胞角蛋白7（CK 7）和α-平滑肌肌动蛋白（α-SMA）的一级抗体。巢蛋白的表达一致地发现在内皮细胞，很少遇到周细胞，假定的基质干/祖细胞，以及在腺上皮细胞。此外，SM的骨膜层中的编织骨形成也可以被视为该膜的成骨潜力的证据。巢蛋白和CD 45在原代骨细胞中的表达支持SM巢蛋白表达细胞的成骨潜能，并支持造血干细胞可能参与上颌窦底重塑。CD 146是一种已知的上皮-间质转化（EMT）诱导剂，在上皮细胞中表达，CK 7也是如此。发现分离的基质细胞表达CD 146、CK 7和α-SMA，表明SM中发生的再生过程也可能涉及产生干/祖细胞的EMT过程。这项研究为施奈德膜的再生潜力提供了额外的证据，并确定了其干细胞龛在成骨中的潜在作用。(C)2015 Wiley Periodicals，Inc.
An innate osteogenic potential of the Schneiderian membrane (SM) is progressively assessed in studies ranging from non-human species to human subjects. It has relevance for endosteal placement and osseointegration. Nestin-expressing osteogenic progenitor cells are allegedly involved in bone formation and remodelling. Nestin phenotype was not assessed previously in human SM. We therefore aimed to fill that particular gap in the literature. Bioptic samples of human adult SM were obtained during surgery from eight adult patients, operated for nonmalignant pathologies. Immunohistochemistry on paraffin-embedded tissue samples used primary antibodies against nestin, CD45, CD146, cytokeratin 7 (CK7), and alpha-smooth muscle actin (alpha-SMA). Nestin expression was consistently found in endothelial cells, and was scarcely encountered in pericytes, putative stromal stem/progenitor cells, as well as in glandular epithelial cells. Moreover, woven bone formation in the periosteal layer of the SM can also be regarded as evidence of the osteogenic potential of this membrane. Nestin and CD45 expression in cells of the primary bone supports the osteogenic potential of SM nestin-expressing cells and a possible involvement of hematopoietic stem cells in maxillary sinus floor remodeling. CD146, a known inducer of epithelial-mesenchymal transition (EMT), was expressed in epithelia, as was CK7. Isolated stromal cells were found expressing CD146, CK7 and alpha-SMA, suggesting that regenerative processes happening in the SM may also involve processes of EMT which generate stem/progenitor cells. This study provides additional evidence for the regenerative potential of the Schneiderian membrane and identifies potential roles for cells of its stem niche in osteogenesis. (C) 2015 Wiley Periodicals, Inc.