Immunohistochemical expression of endothelial markers CD31, CD34, von Willebrand factor, and Fli-1 in normal human tissues
Immunohistochemical expression of endothelial markers CD31, CD34, von Willebrand factor, and Fli-1 in normal human tissues
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DOI：
10.1369/jhc.4a6514.2005
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发表时间：
2006-04-01
影响因子：
3.2
通讯作者：
Bosman, FT
中科院分区：
文献类型：
--
作者：
Pusztaszeri, MP;Seelentag, W;Bosman, FT
很少有系统研究比较内皮细胞(EC)标志物在正常人体组织中不同血管床的表达和分布。采用免疫组织化学方法检测CD31、CD34、von Willebrand因子(VWF)和Fli-1在EC中的表达。从尸检和活检标本中获得的组织样本进行常规处理，并进行CD31、CD34和vWF的免疫组织化学染色。活检组织用免疫组织化学方法检测Fli-1、D2-40和LYVE-1。在所研究的一些器官中，这些标记的表达模式是不同的。肾小球开窗内皮细胞CD31、CD34强表达，vWF仅局限性阳性或完全阴性。肺泡壁毛细血管CD31和CD34强阳性，vWF阴性。随着肺内血管口径的增加，vWF的染色强度逐渐增强。脾、肝窦CD31呈弥漫性阳性。CD34在脾组织中呈阴性表达，而在肝脏中仅在门脉周围区域表达。Fli-1在所有类型的EC中均有表达，在淋巴细胞中也有表达。仅D2-40染色的淋巴管内皮细胞。Lyve-1免疫染色的变异性太大，不能应用于常规处理的组织。EC标记物CD31、CD34和vWF在血管树中的表达是不同的，对于不同的血管类型和同一器官的不同解剖间隔具有特定的模式。D2-40仅标记淋巴管EC。
Few systematic studies have been published comparing the expression and distribution of endothelial cell (EC) markers in different vascular beds in normal human tissues. We investigated by immunohistochemistry the expression of CD31, CD34, von Willebrand factor (vWF), and Fli-1 in EC of the major organs and large vessels. Tissue samples obtained from autopsies and biopsy specimens were routinely processed and stained immunohistochemically for CD31, CD34, and vWF. Biopsy material was also stained immunohistochemically for Fli-1, D2-40, and Lyve-1. The expression pattern of the markers was heterogeneous in some of the organs studied. In the kidney, fenestrated endothelium of the glomeruli strongly expressed CD31 and CD34 but was only focally positive or completely negative for vWF. Alveolar wall capillaries of the lung strongly stained for CD31 and CD34 but were usually negative for vWF. The staining intensity for vWF increased gradually with the vessel caliber in the lung. Sinusoids of the spleen and liver were diffusely positive for CD31. They were negative for CD34 in the spleen and only expressed CD34 in the periportal area in the liver. Fli-1 was expressed in all types of EC but also in lymphocytes. D2-40 stained lymphatic endothelium only. Lyve-1 immunostaining was too variable to be applied to routinely processed tissues. The expression of EC markers CD31, CD34, and vWF in the vascular tree is heterogeneous with a specific pattern for individual vessel types and different anatomic compartments of the same organ. D2-40 labels lymphatic EC only.