Ets‐1: a plausible marker of invasive potential and lymph node metastasis in human oral squamous cell carcinomas
Ets‐1: a plausible marker of invasive potential and lymph node metastasis in human oral squamous cell carcinomas
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Ets-1：人类口腔鳞状细胞癌侵袭潜力和淋巴结转移的合理标志物
作者：
P. Pande;M. Mathur;N. Shukla;R. Ralhan
原癌基因Ets-1是一种转录因子，已知可调节参与细胞外基质重塑的许多基因的表达。据推测，它在血管生成、细胞迁移和调节肿瘤侵袭中发挥作用。在槟榔和烟草相关的口腔肿瘤发生中，预后不良的口腔鳞状细胞癌（SCC）通常表现出广泛的局部疾病，并扩散到区域淋巴结。目前，没有肿瘤标志物可用于预测口腔癌的转移潜力。为了阐明Ets-1在人类口腔肿瘤发生中的作用，我们对60例口腔鳞状细胞癌、30例潜在恶性病变和30例正常口腔粘膜样本进行了免疫组织化学检查，并与免疫印迹法进行了证实。在口腔SCC中确定Ets-1表达与分化程度、淋巴结转移和肿瘤分期的关系。Ets-1在正常口腔上皮中未检测到。在60例口腔鳞状细胞癌中，35例（58%）在肿瘤细胞中显示Ets-1蛋白阳性染色。在潜在恶性病变中，30例病例中有14例（47%）显示Ets-1免疫阳性。Ets-1表达与肿瘤分期（p= 0.01）和淋巴结转移（p= 0.005）显著相关。通过逆转录-聚合酶链反应（RT-PCR）证实了口腔SCC中Ets-1 mRNA的存在。这些发现表明，Ets-1在潜在恶性病变中的过表达可能与血管生成有关，其在口腔SCC中的表达可能是参与肿瘤进展和淋巴结转移的因素之一。版权所有© 1999年约翰威利父子有限公司。
The proto‐oncogene Ets‐1 is a transcription factor known to regulate the expression of a number of genes involved in extracellular matrix remodelling. It has been postulated to play a role in angiogenesis, cell migration, and regulation of tumour invasion. In betel and tobacco‐related oral tumourigenesis, oral squamous cell carcinomas (SCCs) having a poor prognosis often exhibit extensive local disease with spread into regional lymph nodes. At present, no tumour marker is available to predict the metastatic potential of oral carcinomas. To elucidate the involvement of Ets‐1 in human oral tumourigenesis, 60 cases of oral SCC, 30 potentially malignant lesions, and 30 samples of normal oral mucosa were examined by immunohistochemistry and the findings corroborated with immunoblotting. The relationship of Ets‐1 expression with the degree of differentiation, lymph node metastasis, and tumour stage was determined in oral SCCs. Ets‐1 was not detected in the normal oral epithelium. Among the 60 cases of oral SCC, 35 cases (58 per cent) showed positive staining for the Ets‐1 protein in tumour cells. In potentially malignant lesions, 14 of 30 cases (47 per cent) showed Ets‐1 immunopositivity. Ets‐1 expression correlated significantly with tumour stage ( p=0·01) and lymph node metastasis ( p=0·005). The presence of Ets‐1 mRNA in oral SCCs was confirmed by reverse transcription‐polymerase chain reaction (RT‐PCR). These findings suggest that overexpression of Ets‐1 in potentially malignant lesions may be related to angiogenesis and its expression in oral SCCs may be one of the factors involved in tumour progression and lymph node metastasis. Copyright © 1999 John Wiley & Sons, Ltd.
溶基质素/反式蛋白和肿瘤进展。
影响因子：
14.5
作者：
Matrisian,LM;Bowden,GT
通讯作者：
Bowden,GT