Evidence of recombination among enteroviruses
Evidence of recombination among enteroviruses
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DOI：
10.1128/jvi.73.10.8741-8749.1999
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发表时间：
1999-10-01
影响因子：
5.4
通讯作者：
Salminen, M
中科院分区：
文献类型：
--
作者：
Santti, J;Hyypiä, T;Salminen, M
人类肠道病毒由60多种血清型组成，反映了广泛的进化差异。基于单链RNA基因组编码区的序列同源性，它们在遗传上被分为四个簇。为了进一步探索人类肠道病毒之间的遗传关系，并表征负责变异的进化机制，以前测序的基因组进行了详细的比较。Bootstrap和遗传相似性分析用于系统地扫描完整基因组序列的比对。Bootstrap分析提供了来自当前簇的祖细胞的5'非编码区和编码区的接合处的早期重组事件的证据。遗传簇内的分析表明，肠道病毒原型株包括种内重组体。在除衣壳蛋白编码区以外的所有基因组区域中检测到了突变断点。我们的研究结果表明，重组是一个重要的和相对频繁的机制在肠道病毒基因组的进化。
Human enteroviruses consist of more than 60 serotypes, reflecting a wide range of evolutionary divergence. They have been genetically classified into four clusters on the basis of sequence homology in the coding region of the single-stranded RNA genome. To explore further the genetic relationships between human enteroviruses and to characterize the evolutionary mechanisms responsible for variation, previously sequenced genomes were subjected to detailed comparison. Bootstrap and genetic similarity analyses were used to systematically scan the alignments of complete genomic sequences. Bootstrap analysis provided evidence from an early recombination event at the junction of the 5' noncoding and coding regions of the progenitors of the current clusters. Analysis within the genetic clusters indicated that enterovirus prototype strains include intraspecies recombinants. Recombination breakpoints were detected in all genomic regions except the capsid protein coding region. Our results suggest that recombination is a significant and relatively frequent mechanism in the evolution of enterovirus genomes.