Development of a photothermal absorbance detector for use with microfluidic devices.
Development of a photothermal absorbance detector for use with microfluidic devices.
复制标题
开发用于微流体装置的光热吸收检测器。
DOI：
10.1021/ac902975r
复制
发表时间：
2010
影响因子：
7.4
通讯作者：
Jorgenson,JamesW
中科院分区：
文献类型：
--
作者：
Dennis,PattyJ;Welch,ErinFerguson;Alarie,JeanPierre;Ramsey,JMichael;Jorgenson,JamesW
描述了用于微流体装置的光热吸收检测器的开发。与依赖于测量折射率变化的热光技术不同，溶液粘度是通过连续监测溶液电导率来探测的。在石英基板上微加工并粘合到包含微通道的基板上的铂电极可以进行接触式电导率测量。通过实验评估了激励频率和电压、电极间距、激光功率和激光调制（斩波）频率的影响。在当前配置中，使用长注射获得 DABSYL 标记的葡萄糖胺的检测限为 5 nM（以获得平顶峰）。这对应于 4.4 × 10−7AU 的吸光度。 DABSYL 标记的甘氨酸、脯氨酸和色氨酸的分离和检测也证明了该方法的可行性。此外，还使用模拟来研究该技术对小体积平台的适用性。
The development of a photothermal absorbance detector for use with microfluidic devices is described. Unlike thermo-optical techniques that rely on measuring refractive index changes, the solution viscosity is probed by continuously monitoring solution conductivity. Platinum electrodes microfabricated on a quartz substrate and bonded to a substrate containing the microchannels enable contact conductivity measurements. The effects of excitation frequency and voltage, electrode spacing, laser power, and laser modulation (chopping) frequency were evaluated experimentally. In the current configuration, a limit of detection of 5 nM for DABSYL-tagged glucosamine was obtained using long injections (to give flat-topped peaks). This corresponds to an absorbance of 4.4 × 10−7AU. Separation and detection of DABSYL-tagged glycine, proline, and tryptophan are also shown to demonstrate the feasibility of the method. In addition, simulations were used to investigate the applicability of the technique to small volume platforms.