A Workflow Guide to RNA-seq Analysis of Chaperone Function and Beyond.
A Workflow Guide to RNA-seq Analysis of Chaperone Function and Beyond.
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DOI：
10.1007/978-1-4939-7477-1_18
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发表时间：
2018
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影响因子：
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通讯作者：
Calderwood SK
中科院分区：
文献类型：
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作者：
Lang BJ;Holton KM;Gong J;Calderwood SK
RNA 测序 (RNA-seq) 是一种强大的转录本分析方法，可对细胞转录本进行序列识别和定量。 RNA-seq 有许多应用，包括差异基因表达 (DE) 分析、基因融合检测、等位基因特异性表达、异构体和剪接变体定量以及新基因的鉴定。这些应用程序可用于下游系统生物学分析，例如基因本体分析，以深入了解生物条件之间改变的细胞过程。鉴于广泛的信号通路受到伴侣蛋白活性的影响以及 RNA 代谢中众多伴侣蛋白的功能，RNA-seq 可能为生物学和疾病中伴侣蛋白的研究提供有价值的工具。本章概述了一个示例 RNA-seq 工作流程，用于确定两个或多个样本条件之间的差异表达 (DE) 基因，并提供了 RNA-seq 实验设计的一些注意事项。
RNA sequencing (RNA-seq) is a powerful method of transcript analysis that allows for the sequence identification and quantification of cellular transcripts. RNA-seq has many applications including differential gene expression (DE) analysis, gene fusion detection, allele-specific expression, isoform and splice variant quantification and identification of novel genes. These applications can be used for downstream systems biology analyses such as gene ontology analysis to provide insight into cellular processes altered between biological conditions. Given the wide range of signaling pathways subject to chaperone activity as well numerous chaperone functions in RNA metabolism, RNA-seq may provide a valuable tool for study of chaperone proteins in biology and disease. This chapter outlines an example RNA-seq workflow to determine differentially expressed (DE) genes between two or more sample conditions and provides some considerations for RNA-seq experimental design.