Brainstem circuits encoding start, speed, and duration of swimming in adult zebrafish
Brainstem circuits encoding start, speed, and duration of swimming in adult zebrafish
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DOI：
10.1016/j.neuron.2022.10.034
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发表时间：
2023-02-01
期刊：
影响因子：
16.2
通讯作者：
El Manira, Abdeljabbar
中科院分区：
文献类型：
--
作者：
Berg, Eva M.;Mrowka, Leander;El Manira, Abdeljabbar
运动动作的灵活性需要对其开始、持续时间和速度进行精确控制。脑干电路如何编码和传递这些运动参数仍不清楚。在这里，我们结合了体内钙成像、电生理学、解剖学和成年斑马鱼的行为来解决这些问题。我们发现，运动运动的详细参数是由内侧纵束核内两个分子上、结构上和功能上分离的谷氨酸能神经元亚群编码的。运动速度的开始、持续和变化由vGlut2+神经元编码，而vGlut1+神经元编码高速/高幅度运动的突然变化。损毁vGlut2+神经元损害了慢探索游泳，而vGlut1+神经元损毁则损害了快游泳。我们的结果为不同的脑干亚群如何实现灵活的运动指令提供了机械性的见解。这两个脑干指挥亚群被适当地组织起来，以整合环境线索，从而产生灵活的游泳动作，以匹配动物的行为需求。
The flexibility of locomotor movements requires an accurate control of their start, duration, and speed. How brainstem circuits encode and convey these locomotor parameters remains unclear. Here, we have com-bined in vivo calcium imaging, electrophysiology, anatomy, and behavior in adult zebrafish to address these questions. We reveal that the detailed parameters of locomotor movements are encoded by two molecularly, topographically, and functionally segregated glutamatergic neuron subpopulations within the nucleus of the medial longitudinal fasciculus. The start, duration, and changes of locomotion speed are encoded by vGlut2+ neurons, whereas vGlut1+ neurons encode sudden changes to high speed/high amplitude movements. Abla-tion of vGlut2+ neurons compromised slow-explorative swimming, whereas vGlut1+ neuron ablation impaired fast swimming. Our results provide mechanistic insights into how separate brainstem subpopulations imple-ment flexible locomotor commands. These two brainstem command subpopulations are suitably organized to integrate environmental cues and hence generate flexible swimming movements to match the animal's behavioral needs.