An abundant supply of amino acids enhances the metabolic effect of exercise on muscle protein
An abundant supply of amino acids enhances the metabolic effect of exercise on muscle protein
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DOI：
10.1152/ajpendo.1997.273.1.e122
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发表时间：
1997-07-01
影响因子：
5.1
通讯作者：
Wolfe, RR
中科院分区：
文献类型：
--
作者：
Biolo, G;Tipton, KD;Wolfe, RR
对6名未经训练的正常男性进行了研究，在安静时和腿部阻力训练后静脉输注平衡氨基酸混合物（类似于0.15 g.kg（-1）.h（-1），持续3小时），以测试运动对高氨基酸血症引起的肌肉蛋白动力学调节的影响。腿肌肉蛋白质动力学和运输选定的氨基酸（丙氨酸，苯丙氨酸，亮氨酸和赖氨酸）的同位素确定使用的模型的基础上动静脉血样和肌肉活检。静脉输注氨基酸导致休息时和运动后动脉氨基酸浓度相当的增加，而运动后腿部血流量比休息时高64 +/- 5%。在高氨基酸血症期间，运动后氨基酸转运高于基础水平的增加比休息时大30-100%。运动后肌肉蛋白质合成的增加也大于休息时（291 +/- 42% vs. 141 +/- 45%）。肌肉蛋白质分解没有显着影响高氨基酸血症，无论是在休息或运动后。我们的结论是，外源性氨基酸对肌肉蛋白质合成的刺激作用增强事先锻炼，也许部分是因为增强血流我们的研究结果表明，运动后立即摄入的蛋白质可能比在稍后的时间摄入更多的合成代谢。
Six normal untrained men were studied during the intravenous infusion of a balanced amino acid mixture (similar to 0.15 g.kg(-1).h(-1) for 3h) at rest and after a leg resistance exercise routine to test the influence of exercise on the regulation of muscle protein kinetics by hyperaminoacidemia. Leg muscle protein kinetics and transport of selected amino acids (alanine, phenylalanine, leucine, and lysine) were isotopically determined using a model based on arteriovenous blood samples and muscle biopsy. The intravenous amino acid infusion resulted in comparable increases in arterial amino acid concentrations at rest and after exercise, whereas leg blood flow was 64 +/- 5% greater after exercise than at rest. During hyperaminoacidemia, the increases in amino acid transport above basal were 30-100% greater after exercise than at rest. Increases in muscle protein synthesis were also greater after exercise than at rest (291 +/- 42% vs. 141 +/- 45%). Muscle protein breakdown was not significantly affected by hyperaminoacidemia either at rest or after exercise. We conclude that the stimulatory effect of exogenous amino acids on muscle protein synthesis is enhanced by prior exercise, perhaps in part because of enhanced blood flow Our results imply that protein intake immediately after exercise may be more anabolic than when ingested at some later time.