Rapid developmental switch in the mechanisms driving early cortical columnar networks
Rapid developmental switch in the mechanisms driving early cortical columnar networks
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DOI：
10.1038/nature04264
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发表时间：
2006-01-05
期刊：
影响因子：
64.8
通讯作者：
Luhmann, HJ
中科院分区：
文献类型：
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作者：
Dupont, E;Hanganu, IL;Luhmann, HJ
未成熟的大脑皮层早在被模式化的感觉输入激活之前就已自组织成局部神经元簇。在新生哺乳动物的皮质原基中，神经元通过电突触或化学突触自发地 (2-4) 或通过递质门控受体的激活共同组装 (5,6)。早期发育过程中皮层自组织过程背后的神经元网络和细胞机制尚未完全了解。在这里，我们在未成熟小鼠大脑皮层的完整体外制备中展示了神经元通过在β频率范围内传播网络振荡在局部簇中功能耦合。在新生小鼠中，这种活动需要完整的亚板，并且通过间隙连接在皮质柱内强烈同步。随着出生后第一周末亚板的发育消失 (7)，未成熟皮质网络中 NMDA（N-甲基-D-天冬氨酸）受体的激活对于产生这种柱状活动模式至关重要。我们的研究结果表明，在短暂的发育时期，皮质网络从亚板驱动、间隙连接耦合的合胞体转变为通过 NMDA 受体起作用的突触网络，以产生同步振荡活动，这可能作为皮质柱状结构发育的早期功能模板。
The immature cerebral cortex self-organizes into local neuronal clusters long before it is activated by patterned sensory inputs1. In the cortical anlage of newborn mammals, neurons coassemble through electrical or chemical synapses either spontaneously(2-4) or by activation of transmitter-gated receptors(5,6). The neuronal network and the cellular mechanisms underlying this cortical self-organization process during early development are not completely understood. Here we show in an intact in vitro preparation of the immature mouse cerebral cortex that neurons are functionally coupled in local clusters by means of propagating network oscillations in the beta frequency range. In the newborn mouse, this activity requires an intact subplate and is strongly synchronized within a cortical column by gap junctions. With the developmental disappearance of the subplate at the end of the first postnatal week(7), activation of NMDA (N-methyl-D-aspartate) receptors in the immature cortical network is essential to generate this columnar activity pattern. Our findings show that during a brief developmental period the cortical network switches from a subplate-driven, gap-junction-coupled syncytium to a synaptic network acting through NMDA receptors to generate synchronized oscillatory activity, which may function as an early functional template for the development of the cortical columnar architecture.