Higher-level production of ascomycin (FK520) by Streptomyces hygroscopicus var. ascomyceticus irradiated by femtosecond laser
Higher-level production of ascomycin (FK520) by Streptomyces hygroscopicus var. ascomyceticus irradiated by femtosecond laser
复制标题
吸水链霉菌变种更高水平地生产子囊霉素 (FK520)。
DOI：
10.1007/s12257-012-0114-2
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发表时间：
2012-08-01
影响因子：
3.2
通讯作者：
Jia, Xiao-qiang
中科院分区：
文献类型：
--
作者：
Qi, Hai-shan;Xin, Xing;Jia, Xiao-qiang
首次采用飞秒激光辐照技术提高吸水链霉菌FK 520的产量。子囊菌NT 2 -11是一株经N-甲基-N-硝基-N-亚硝基胍（NTG）诱导的来源于S.吸水菌（ATCC 14891）。采用钛蓝宝石激光系统（810 nm，76 MHz，150 fs），在最佳照射条件（25 mW，6 min）下，获得了FK 520高产稳定突变株FS 35。FS 35的FK 520生产能力比亲本菌株NT 2 -11提高了45%。在优化的发酵条件下，FS 35的FK 520发酵效价达到300 mg/L，并对FS 35和NT 2 -11的3个阶段的本征动力学进行了比较研究。该数学模型对FK 520发酵过程进行了较好的描述，为FK 520发酵过程的优化操作和中试放大研究提供了有价值的信息。对模型参数的比较研究证实了飞秒激光照射在生产和降低底物抑制方面的优势。因此，飞秒激光辐照为提高FK 520在S.吸湿性。
Femtosecond laser irradiation technology was employed for the first time to improve the ascomycin (FK520) yield of Streptomyces hygroscopicus var. ascomyceticus NT2-11, which is an N-methyl-N-nitro-N-nitrosoguanidine (NTG)-induced strain derived from S. hygroscopicus (ATCC14891). The mutant FS35 with high and stable FK520 production capacity was then obtained in the optimal irradiation conditions (25 mW for 6 min) by the Titanium sapphire laser system (810 nm, 76 MHz, 150 fs). The FK520 production capacity of FS35 was 45% higher than that of the parental strain NT2-11. Moreover, under the optimal fermentation conditions, FK520 fermentation titer of FS35 reached 300 mg/L and the intrinsic kinetics of FS35 and NT2-11 were investigated comparatively in 3 phases. The mathematical models provided a good description of FK520 fermentation process for both strains and valuable information for optimizing operation and pilotplant enlargement research. The comparative studies on parameters of the models confirmed the advantages in production and the decrease of substrate inhibition through femtosecond laser irradiation. Therefore, femtosecond laser irradiation provides a promising way to enhance the production of FK520 in S. hygroscopicus.