Transitions in RNA polymerase II elongation complexes at the 3′ ends of genes
Transitions in RNA polymerase II elongation complexes at the 3′ ends of genes
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DOI：
10.1038/sj.emboj.7600053
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发表时间：
2004-01-28
期刊：
影响因子：
11.4
通讯作者：
Buratowski, S
中科院分区：
文献类型：
--
作者：
Kim, M;Ahn, SH;Buratowski, S
为了了解体内转录 RNA 聚合酶 II (RNApII) 的因子相互作用，使用染色质免疫沉淀来绘制跨转录基因的多个延伸和聚腺苷酸化因子的交联模式。通过聚腺苷酸化位点的转录会导致 Ctk1 激酶水平及其相关的 RNApII C 末端结构域磷酸化水平降低。一组延伸因子（Spt4/5、Spt6/Iws1 和 Spt16/Pob3）被认为通过染色质介导转录，显示出与 RNApII 匹配的模式。相反，Paf 和 TREX/THO 复合物部分重叠 RNApII，但不与多腺苷酸化位点下游的转录区域交联。在互补模式中，聚腺苷酸化因子在基因的 30 端强烈交联。 30 个聚腺苷酸化序列或 Rna14 蛋白的突变会导致聚腺苷酸化因子交联和终止序列通读的丧失。因此，转录终止和聚腺苷酸化涉及基因 30 末端的转变，其中可能包括延伸和聚腺苷酸化/终止因子的交换。
To understand the factor interactions of transcribing RNA polymerase II (RNApII) in vivo, chromatin immunoprecipitations were used to map the crosslinking patterns of multiple elongation and polyadenylation factors across transcribed genes. Transcription through the polyadenylation site leads to a reduction in the levels of the Ctk1 kinase and its associated phosphorylation of the RNApII C-terminal domain. One group of elongation factors (Spt4/5, Spt6/Iws1, and Spt16/Pob3), thought to mediate transcription through chromatin, shows patterns matching that of RNApII. In contrast, the Paf and TREX/THO complexes partially overlap RNApII, but do not crosslink to transcribed regions downstream of polyadenylation sites. In a complementary pattern, polyadenylation factors crosslink strongly at the 30 ends of genes. Mutation of the 30 polyadenylation sequences or the Rna14 protein causes loss of polyadenylation factor crosslinking and readthrough of termination sequences. Therefore, transcription termination and polyadenylation involve transitions at the 30 end of genes that may include an exchange of elongation and polyadenylation/ termination factors.