Thermomicrofluidics for biosensing applications
Thermomicrofluidics for biosensing applications
复制标题
生物传感应用的热微流体
DOI：
10.1002/viw.20200148
复制
发表时间：
2021-05
期刊：
影响因子：
8.6
通讯作者：
Fei Tian;Ziwei Han;Jinqi Deng;Chao Liu;Jiashu Sun
中科院分区：
文献类型：
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作者：
Fei Tian;Ziwei Han;Jinqi Deng;Chao Liu;Jiashu Sun
对含有生物分子和生物制品的生物体系的准确检测在各种研究领域中越来越重要，特别是在分析和生物化学领域。在微流控装置(热微流控)中实施热电泳法能够以无标记和高精度的方式操纵和测量生物分子和生物制品，为生物传感提供了一条很有前途的途径。本文介绍了微流控系统中热电泳法的基本原理及其与其他热诱导物理现象的耦合。我们概述了热微流体在不同的生物传感应用中的能力，如生物分子相互作用的监测、核酸的检测、细胞外小泡的分析和细胞的操纵。热微流体生物传感技术提供了对生理病理过程和疾病诊断的洞察。讨论了目前热微流控检测面临的挑战和进一步发展的方向。
The accurate detection of biological systems containing biomolecules and bioparticles is increasingly important in diverse research fields, particularly in analytical and biological chemistry. Implementing thermophoresis in microfluidic devices (thermomicrofluidics) enables the manipulation and measurement of biomolecules and bioparticles in a label‐free and high‐precision manner, providing a promising avenue for biosensing. This review presents fundamentals of thermophoresis and its coupling with other thermal‐induced physical phenomena in the microfluidic setups. We overview the capabilities of thermomicrofluidics for diverse biosensing applications such as monitoring of biomolecular interactions, detection of nucleic acids, profiling of extracellular vesicles, and manipulation of cells. Biosensing by thermomicrofluidics provides insights into physiopathological processes and disease diagnostics. Current challenges and further directions of thermomicrofluidic detection are discussed.