Role of Scrib and Dlg in anterior-posterior patterning of the follicular epithelium during Drosophila oogenesis.
Role of Scrib and Dlg in anterior-posterior patterning of the follicular epithelium during Drosophila oogenesis.
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Scrib 和 Dlg 在果蝇卵子发生过程中滤泡上皮前后模式的作用
DOI：
10.1186/1471-213x-9-60
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发表时间：
2009-12-01
影响因子：
--
通讯作者：
Li M
中科院分区：
文献类型：
--
作者：
Li Q;Shen L;Xin T;Xiang W;Chen W;Gao Y;Zhu M;Yu L;Li M
背景：卵泡细胞上皮在卵腔上的正确模式对果蝇卵的发育至关重要。上皮沿前后轴分化为几种不同的细胞类型需要多种信号通路的协调活动。在此之前，我们报道了致死性(2)巨型幼虫（lgl），一种果蝇肿瘤抑制基因，需要在卵泡细胞中诱导卵发生中期的后卵泡细胞（PFC）命运。在这里，我们探讨了另外两个肿瘤抑制基因scribble （scrib）和discs large （dlg）在上皮模式中的作用。结果：我们发现去除卵腔后端卵泡细胞的scrib或dlg功能会导致PFC命运的完全丧失。嵌合克隆中pfc的异常分化可归因于多层细胞中EGFR、JAK和Notch信号通路协调激活的缺陷。与此同时，克隆分析显示，scrib/dlg前域的功能缺失突变导致伸展和向心性细胞命运缺陷诱导的部分渗透表型，而边缘细胞命运的特异性仍然可以发生在突变克隆的大多数前区域。此外，我们发现scrib基因与dlg相互作用，调节上皮后模式。结论：在本研究中，我们提供了scrib和dlg在卵泡上皮前、后形态上的功能差异的证据。进一步的遗传分析表明scrib和dlg在一个共同的途径中调控PFC的命运诱导。该研究可能为阐明scrib/dlg在发育过程中调控上皮极性和细胞增殖中的作用打开了另一扇窗口。
Background:Proper patterning of the follicle cell epithelium over the egg chamber is essential for the Drosophila egg development. Differentiation of the epithelium into several distinct cell types along the anterior-posterior axis requires coordinated activities of multiple signaling pathways. Previously, we reported that lethal(2)giant larvae (lgl), a Drosophila tumor suppressor gene, is required in the follicle cells for the posterior follicle cell (PFC) fate induction at mid-oogenesis. Here we explore the role of another two tumor suppressor genes, scribble (scrib) and discs large (dlg), in the epithelial patterning.Results:We found that removal of scrib or dlg function from the follicle cells at posterior terminal of the egg chamber causes a complete loss of the PFC fate. Aberrant specification and differentiation of the PFCs in the mosaic clones can be ascribed to defects in coordinated activation of the EGFR, JAK and Notch signaling pathways in the multilayered cells. Meanwhile, the clonal analysis revealed that loss-of-function mutations in scrib/dlg at the anterior domains result in a partially penetrant phenotype of defective induction of the stretched and centripetal cell fate, whereas specification of the border cell fate can still occur in the most anterior region of the mutant clones. Further, we showed that scrib genetically interacts with dlg in regulating posterior patterning of the epithelium.Conclusion:In this study we provide evidence that scrib and dlg function differentially in anterior and posterior patterning of the follicular epithelium at oogenesis. Further genetic analysis indicates that scrib and dlg act in a common pathway to regulate PFC fate induction. This study may open another window for elucidating role of scrib/dlg in controlling epithelial polarity and cell proliferation during development.