Cytoplasmic and nuclear progestin receptors in human myometrium during the menstrual cycle and in pregnancy at term.
Cytoplasmic and nuclear progestin receptors in human myometrium during the menstrual cycle and in pregnancy at term.
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月经周期和足月妊娠期间人类子宫肌层中的细胞质和核孕激素受体。
DOI：
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发表时间：
1979
影响因子：
5.8
通讯作者：
D. Tulchinsky
中科院分区：
文献类型：
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作者：
G. Giannopoulos;D. Tulchinsky
已开发出交换测定法，用于使用放射性标记的 R5020（17,21-二甲基-19-去甲-4,9-孕二烯-3,20-二酮）作为配体测定人子宫肌层细胞质和细胞核中受体位点（未占据和被内源激素占据）中的总孕激素。这些测定已应用于测量月经周期和足月妊娠期间子宫肌层中的胞质和核孕激素受体位点。细胞质受体水平以及细胞质和核受体水平之和在周期的增殖期最高，在分泌期适度但显着下降，并在足月妊娠时显着下降。尽管胞质受体水平显着下降，但足月妊娠时的核受体水平与增殖或分泌期观察到的相似。此外，与细胞核相关的总细胞受体的比例从增殖期的 5.5% 增加到足月妊娠时的 60%。足月妊娠时胞浆孕激素受体浓度的降低以及受体在人类子宫肌层核定位的相对增加可能与高循环孕酮水平有关。
Exchange assays have been developed for the determination of the total progestin in receptor sites (unoccupied and occupied with endogenous hormone) in the cytosol and nuclei of human myometrium using radiolabeled R5020 (17,21-dimethyl-19-nor-4,9-pregnadiene-3,20-dione) as ligand. These assays have been applied to the measurement of cytosol and nuclear progestin receptor sites in the myometrium during the menstrual cycle and in pregnancy at term. Both the cytosol receptor levels as well as the sum of cytosol and nuclear receptor levels were highest during the proliferative phase of the cycle, dropped moderately but significantly in the secretory phase, and decreased markedly in pregnancy at term. Despite the marked drop in cytosol receptor levels, nuclear receptor levels in pregnancy at term were similar to those observed in the proliferative or secretory phase. In addition, the fraction of the total cellular receptor that was associated with the nucleus increased from 5.5% in the proliferative phase to 60% in pregnancy at term. The decrease in cytosol progestin receptor concentration as well as the relative increase in nuclear localization of the receptor in human myometrium in pregnancy at term may be related to the high circulating progesterone levels.