Infection of Human Retinal Pigment Epithelium with Chlamydia trachomatis
Infection of Human Retinal Pigment Epithelium with Chlamydia trachomatis
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DOI：
10.1371/journal.pone.0141754
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发表时间：
2015-11-04
期刊：
影响因子：
3.7
通讯作者：
Suetov, Alexei
中科院分区：
文献类型：
--
作者：
Boiko, Ernest;Maltsev, Dmitrii;Suetov, Alexei
目的沙眼衣原体感染后段组织，特别是人视网膜色素上皮(HRPE)对沙眼衣原体的敏感性知之甚少。本研究旨在探讨沙眼衣原体感染hRPE的可能性，并检测不同沙眼衣原体临床分离株对hRPE细胞的感染力及细胞对感染的反应。方法将8株沙眼衣原体临床分离株以2.0或0.3的复数感染hRPE和McCoy细胞。分别于接种后24小时、48小时和72小时用抗沙眼衣原体主要外膜蛋白抗体进行免疫组织化学染色以检测沙眼衣原体。结果8株临床分离株均具有感染hRPE细胞的能力。在相同MOI为0.3时，沙眼衣原体临床分离株对RPE培养的感染力至少与McCoy细胞培养的一样高。含包涵体细胞百分率在MOI为2.0的hRPE细胞中为1.5+/-0.52~14.6+/-3.3%，而在MOI为0.3的McCoy细胞中为0.37±0.34~8.9+/-0.2%。感染沙眼衣原体的hRPE细胞培养48hI型胶原、IV型胶原、碱性成纤维细胞生长因子、转化生长因子-β和白介素8的表达最高，分别是对照组的2.1倍、1.3倍、1.5倍、1.5倍和1.6倍(P<；0.05)。结论本研究首次证实了沙眼衣原体感染hRPE细胞的可能性，导致细胞信号分子和细胞外基质成分表达的增殖性和促炎性改变。
PurposeLittle is known about the susceptibility of posterior segment tissues, particularly the human retinal pigment epithelium (hRPE), to Chlamydia trachomatis. The purpose of the study was to investigate the possibility of infecting the hRPE with Chlamydia trachomatis, and to examine the infectivity of different Chlamydia trachomatis clinical isolates for hRPE cells and the hRPE cell response to the infection.MethodsCultured hRPE and McCoy cells were inoculated with eight Chlamydia trachomatis (serovar E) clinical isolates at multiplicity of infection (MOI) of 2.0 or 0.3. To detect Chlamydia trachomatis, samples were stained immunohistochemically with anti-major outer membrane protein antibodies at 24h, 48h, and 72h postinoculation (PI). The changes in the expression of signaling molecules and proteins of cytoskeleton and extracellular matrix in hRPE cells were examined immunohistochemically.ResultsAll eight clinical isolates demonstrated ability to infect hRPE cells. At equal MOI of 0.3, the infectivity of Chlamydia trachomatis clinical isolates for RPE culture was found to be at least as high as that for McCoy cell culture. At 24h PI, the percentage of inclusion-containing cells varied from 1.5 +/- 0.52 to 14.6 +/- 3.3% in hRPE cell culture infected at MOI of 2.0 against 0.37 +/- 0.34 to 8.9 +/- 0.2% in McCoy cell culture infected at MOI of 0.3. Collagen type I, collagen type IV, basic fibroblast growth factor, transforming growth factor-beta and interleukin-8 expression at 48h PI were maximally increased, by 2.1-, 1.3-, 1.5-, 1.5- and 1.6-fold, respectively, in the Chlamydia trachomatis-infected compared with control hRPE cell culture specimens (P < 0.05).ConclusionsThis study, for the first time, proved the possibility of infecting hRPE cultured cells with Chlamydia trachomatis, which leads to proproliferative and proinflammatory changes in the expression of signaling molecules and extracellular matrix components.