Human myeloid cell nuclear differentiation antigen binds specifically to nucleolin.
Human myeloid cell nuclear differentiation antigen binds specifically to nucleolin.
复制标题
人骨髓细胞核分化抗原与核仁素特异性结合。
DOI：
10.1002/jcb.240590412
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Briggs,RC
中科院分区：
文献类型：
--
作者：
Xie,J;Briggs,JA;Olson,MO;Sipos,K;Briggs,RC
人髓细胞核分化抗原（MNDA）是一种在髓单核细胞谱系中特异性表达的核蛋白，受干扰素α以细胞特异性方式调节。MNDA也是一个功能未知的干扰素调控基因家族的成员。为了阐明MNDA的功能，采用亲和纯化、共免疫沉淀和蛋白印迹三种技术对其特异性蛋白结合活性进行了表征。微序列分析表明，MNDA与100 kDa的核仁蛋白结合。特异性抗体免疫反应证实了核仁蛋白的鉴定。MNDA含有可以解释与核仁蛋白特异性结合的基序。核蛋白与其他大分子结合，在信号转导、核糖体产生、核基质结构和转录调节等方面表现出一致的特征。目前的结果表明，MNDA的功能很可能与其他蛋白质的相互作用有关。通过这些关联，MNDA可以对普遍存在的蛋白质（如核蛋白）的功能提供细胞/谱系和分化特异性限制。对MNDA蛋白结合的进一步分析可能对阐明MNDA的功能至关重要，并有助于了解该干扰素诱导基因家族其他成员产物的功能。©1995 Wiley‐Liss, Inc。
The human myeloid cell nuclear differentiation antigen (MNDA) is a nuclear protein expressed specifically in cells of the myelomonocytic lineage and regulated by interferon α in a cell‐specific fashion. MNDA is also a member of a family of interferon‐regulated genes of unknown function. In an effort to elucidate the function of MNDA, three techniques (affinity purification, coimmunoprecipitation, and protein blot assay) were used to characterize its specific protein binding activities. Microsequence analysis showed that MNDA bound the 100 kDa nucleolin protein. The identification of nucleolin was confirmed by immunoreaction with specific antibodies. MNDA contains motifs which could account for specific binding to nucleolin. Nucleolin binds other macromolecules and exhibits features consistent with roles in signal transduction, production of ribosomes, nuclear matrix structure, and regulation of transcription. The present results indicate that the function of MNDA is most likely related to interactions with other proteins. Through these associations, MNDA could contribute cell/lineage‐ and differentiation‐specific limits to the function of ubiquitous proteins such as nucleolin. Further analysis of MNDA protein binding could be critical to elucidating the function of MNDA and could contribute to understanding the fuction of the products of other members of this interferon‐inducible family of genes. © 1995 Wiley‐Liss, Inc.