Microfluidic approaches for the fabrication of gradient crosslinked networks based on poly(ethylene glycol) and hyperbranched polymers for manipulation of cell interactions.
Microfluidic approaches for the fabrication of gradient crosslinked networks based on poly(ethylene glycol) and hyperbranched polymers for manipulation of cell interactions.
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DOI：
10.1002/jbm.a.32974
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发表时间：
2011-01
影响因子：
4.9
通讯作者：
Anseth, K. S.
中科院分区：
文献类型：
--
作者：
Pedron, S.;Peinado, C.;Bosch, P.;Benton, J. A.;Anseth, K. S.
关键词：
高通量方法允许快速检查参数空间以表征材料并开发用于生物材料应用的新聚合物制剂。一个限制是难以用常规仪器和样品制备制备文库和进行高通量筛选。在这里，我们描述了基板材料的制造与控制梯度组成的快速方法的微观混合，然后通过光聚合反应。具体地，聚（乙二醇）二甲基丙烯酸酯与超支化多甲基丙烯酸酯（P1000 MA或H30 MA）以梯度方式共聚。甲基丙烯酸酯转化的程度和最终的网络组合物通过近红外光谱法测定，并通过纳米压痕法测量机械性能。弹性模量和网络交联密度之间的关系。粗糙度和亲水性增加的表面与较高浓度的P1000 MA。这些结果可能与超支化大分子聚合物在光聚合反应期间发生的相分离过程有关。另一方面，在H30 MA聚合反应中的最终转化率的降低归因于作为网络软化的结果的较低的终止率。评价瓣膜间质细胞附着在这些梯度基质上作为研究细胞形态作为局部基质性质的函数的证明。数据显示，P1000 MA的存在影响细胞-材料相互作用，具有较高数量的粘附细胞和更多的细胞在具有较高含量的多功能交联剂的梯度区域上扩散。
High-throughput methods allow rapid examination of parameter space to characterize materials and develop new polymeric formulations for biomaterials applications. One limitation is the difficulty of preparing libraries and performing high-throughput screening with conventional instrumentation and sample preparation. Here, we describe the fabrication of substrate materials with controlled gradients in composition by a rapid method of micromixing followed by a photopolymerization reaction. Specifically, poly(ethylene glycol) dimethacrylate was copolymerized with a hyperbranched multimethacrylate (P1000MA or H30MA) in a gradient manner. The extent of methacrylate conversion and the final network composition were determined by near-infrared spectroscopy, and mechanical properties were measured by nanoindentation. A relationship was observed between the elastic modulus and network crosslinking density. Roughness and hydrophilicity were increased on surfaces with a higher concentration of P1000MA. These results likely relate to a phase segregation process of the hyperbranched macromer that occurs during the photopolymerization reaction. On the other hand, the decrease in the final conversion in H30MA polymerization reactions was attributed to the lower termination rate as a consequence of the softening of the network. Valvular interstitial cell attachment was evaluated on these gradient substrates as a demonstration of studying cell morphology as a function of the local substrate properties. Data revealed that the presence of P1000MA affects cell–material interaction with a higher number of adhered cells and more cell spreading on gradient regions with a higher content of the multifunctional crosslinker.
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影响因子：
64.5
作者：
Choquet, D;Felsenfeld, DP;Sheetz, MP
通讯作者：
Sheetz, MP
影响因子：
5.5
作者：
MALMSTROM, E;JOHANSSON, M;HULT, A
通讯作者：
HULT, A
DOI：
10.1073/pnas.0903994106
发表时间：
2009-10-27
影响因子：
11.1
作者：
Mitrossilis, Demosthene;Fouchard, Jonathan;Asnacios, Atef
通讯作者：
Asnacios, Atef
影响因子：
4.6
作者：
Crowe-Willoughby, Julie A.;Weiger, Katherine L.;Genzer, Jan
通讯作者：
Genzer, Jan
影响因子：
--
作者：
Qian, Zhenyu;Minnikanti, Venkatachala S.;Archer, Lynden A.
通讯作者：
Archer, Lynden A.