NERVE GROWTH-FACTOR RECEPTORS ON HUMAN MELANOMA CELLS IN CULTURE
NERVE GROWTH-FACTOR RECEPTORS ON HUMAN MELANOMA CELLS IN CULTURE
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DOI：
10.1073/pnas.74.2.565
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发表时间：
1977-01-01
影响因子：
11.1
通讯作者：
TODARO, GJ
中科院分区：
文献类型：
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作者：
FABRICANT, RN;DELARCO, JE;TODARO, GJ
用放射性核素125I标记纯化的小鼠神经生长因子(NGF)，检测其与多种不同培养细胞的结合能力。NGF很容易与人和仓鼠黑色素瘤细胞结合，但不与许多其他细胞系结合。NGF受体数量最多的3个细胞系来自转移性黑色素瘤。其中一个品系，A875，被详细研究了7次。105个NGF受体/细胞，结合常数为1.0倍。109万L/摩尔。在竞争结合分析中使用这些细胞，可以在各种生物液中检测到0.25 ng的NGF。可以证明NGF可以增加黑色素瘤细胞的存活率，但不能增加黑色素瘤细胞的分裂，这些黑色素瘤细胞维持在缺乏血清生长因子的培养基中。作为一种特殊的生存因子，NGF的这种作用似乎类似于它对培养的交感神经节细胞和其他源自神经脊的细胞的影响。
Purified mouse nerve growth factor (NGF) radiolabeled with 125I was tested for its ability to bind a variety of different cultured cells. NGF binds readily to human and hamster melanoma cells but does not bind to many other cell lines. The 3 cell lines with the highest number of NGF receptors were derived from metastatic melanomas. One of these lines, A875, was studied in detail and had 7 .times. 105 NGF receptors/cell with an association constant of 1.0 .times. 109 l/mol. The use of these cells in competition binding assays permits the detection of 0.25 ng of NGF in various biologic fluids. NGF can be shown to increase the survival, but not the division, of melanoma cells maintained in medium depleted of serum growth factors. This effect of NGF as a specific survival factor appears analogous to its effect on cultured sympathetic ganglion cells and other cells derived from the neural crest.