Characterization of two TCR transgenic mouse lines specific for herpes simplex virus
Characterization of two TCR transgenic mouse lines specific for herpes simplex virus
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DOI：
10.1046/j.1440-1711.2002.01071.x
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发表时间：
2002-04-01
影响因子：
4
通讯作者：
Jones, CM
中科院分区：
文献类型：
--
作者：
Mueller, SN;Heath, WR;Jones, CM
为了更好地了解T细胞介导的1型单纯疱疹病毒（HSV-1）感染的免疫应答过程，我们制备了两种hsv特异性T细胞受体（TCR）转基因小鼠系。这些老鼠（gBT-I）。1和gbt - 1。3)是MHC i类限制性的，对HSV糖蛋白B（gB）的免疫优势肽（gB(498-505)）具有特异性。虽然来自同一克隆，但TCR基因的染色体位置不同，在胸腺CD4（+）和CD8（+）细胞上TCR α / β的表达也有显著差异。尽管如此，来自两种小鼠的外周CD8(+) T细胞表达同样高水平的转基因TCR，并以相同程度结合K-b gB（498-505）四聚体。与此相一致的是，在体外用gB（498-505）肽或单纯疱疹病毒感染的细胞刺激时，两者都表现出同样好的反应。这些数据表明，在存在明显不同的胸腺t细胞亚群的情况下，可以发生大致相同的外周t细胞亚群的选择。
To better understand the T cell-mediated processes involved in the immune response to herpes simplex virus type 1 (HSV-1) infection, two HSV-specific T cell receptor (TCR) transgenic mouse lines were produced. These mice (gBT-I.1 and gBT-I.3) are MHC class I-restricted and specific for the immunodominant peptide from HSV glycoprotein B (gB), gB(498-505) . Although derived from the same clone, the mice differ in the chromosomal location of the TCR transgenes and show marked differences in TCR alpha/beta expression on both CD4(+) and CD8(+) cells in the thymus. Despite this, peripheral CD8(+) T cells from both mice express equally high levels of the transgenic TCR and bind the K-b gB(498-505) tetramer to the same degree. In concordance with this, both were shown to respond equally well in vitro upon stimulation with the gB(498-505) peptide or HSV-infected cells. These data show that selection of broadly equivalent peripheral T-cell subsets can occur in the presence of distinctly different thymic T-cell subsets.