Effects of low-dose oleuropein diet supplementation on the oxidative status of skeletal muscles and plasma hormonal concentration of growing broiler chickens
Effects of low-dose oleuropein diet supplementation on the oxidative status of skeletal muscles and plasma hormonal concentration of growing broiler chickens
复制标题
日粮添加低剂量橄榄苦苷对生长肉鸡骨骼肌氧化状态和血浆激素浓度的影响
DOI：
10.1080/00071668.2019.1662886
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发表时间：
2019
影响因子：
2
通讯作者：
Kikusato M.
中科院分区：
文献类型：
--
作者：
Shimao R.;Muroi H.;Furukawa K.;Toyomizu M.;Kikusato M.
1.橄榄苦苷（Ole）是油橄榄（Olea europaea）中主要的酚类化合物，具有抗氧化、抗肥胖、抗炎等作用。为探讨Ole对家禽生理代谢的影响，本研究评价了低剂量Ole对生长期肉仔鸡生产性能、代谢激素状态、肌肉氧化状态的影响.将32只8日龄鸡随机分为4组，分别饲喂0（对照）、0.1、0.5和2.5ppm油酸添加日粮2周。在试验组之间的饲喂期间，体重增加、摄食量和饲料效率无差异。与对照组相比，饲喂Ole 0.5 ppm和2.5 ppm补充饲料的鸟类肌肉羰基含量显著降低。在喂食Ole 0.5 ppm的组中，线粒体ROS降低因子的mRNA表达水平：禽类解偶联蛋白和锰超氧化物歧化酶，以及过氧化物酶体增殖物激活受体γ共激活因子1-α、sirtuin-1和-3（每一种都协同诱导前两种因子的转录）与对照组相比被上调，其变化与血浆去甲肾上腺素和甲状腺激素水平无关。Ole-2.5 ppm组没有表现出这种转录变化，但表现出更高的皮质酮浓度。这项研究表明，摄入低剂量的Ole可以减少肌肉氧化损伤，并且抑制机制可能取决于生长肉鸡摄入的Ole量。
1. Oleuropein (Ole) is a major phenolic compound inOlea europaea, with anti-oxidative, anti-obesity, and anti-inflammatory properties. To explore the effect of Ole on the physiology and metabolism of poultry, this study, evaluated the effects of feeding low-dose Ole on the growth performance, metabolic hormonal status, muscle oxidative status in growing broiler chickens.2. Thirty-two 8-day-old chickens were assigned to four different treatments, and fed either 0 (control), 0.1, 0.5, or 2.5 ppm Ole-supplemented diets for 2 weeks.3. There were no differences in the body weight gain, feed consumption, and feed efficiency during the feeding periods between the groups tested. Birds fed Ole 0.5- and 2.5 ppm-supplemented diets exhibited a significant decrease in muscle carbonyl content compared to the control group. In the group fed Ole 0.5 ppm, the mRNA expression levels of mitochondrial ROS-reducing factors: avian uncoupling protein and manganese superoxide dismutase, as well as peroxisome proliferator-activated receptor γ coactivator 1-α, sirtuin-1 and −3 (each of which co-ordinately induce the transcription of the previous two factors) were upregulated compared to the control group, and the changes were independent of plasma noradrenaline and thyroid hormone levels. The group fed Ole-2.5 ppm did not show such transcriptional changes, but exhibited a higher corticosterone concentration.4. This study demonstrates that ingesting a low dose of Ole can reduce muscle oxidative damage, and that the suppression machinery may differ depending on the amount of Ole ingested by growing broiler chickens.