Prolonged inhibition of normal ovarian cycles in the rat and cynomolgus monkeys following a single sc injection of danazol
Prolonged inhibition of normal ovarian cycles in the rat and cynomolgus monkeys following a single sc injection of danazol
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DOI：
10.1093/humrep/12.7.1409
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发表时间：
1997-07-01
影响因子：
6.1
通讯作者：
Castracane, VD
中科院分区：
文献类型：
--
作者：
Butterstein, GM;Mann, DR;Castracane, VD
在去势雄性大鼠中，单次s.c.在本研究中，我们已经检查了相同和相似的给药途径是否抑制正常周期大鼠和食蟹猴的卵巢功能。或s.c.每天跟踪直到下一个发情期涂片的发情间期持续时间，并将周期长度与赋形剂处理组进行比较。对6只正常循环的食蟹猴进行每日观察，并以2-3天间隔采血。跟踪猴直到下一次月经和之后的一个周期，并测定血样的雌二醇、孕酮和生物活性LH。溶媒对照组大鼠的发情周期为4.7天。高剂量组使异情期延长至31.3天（P
In castrated male rats, a single s.c. injection of danazol has been shown to result in an inordinately prolonged inhibition of serum luteinizing hormone (LH) and follicle stimulating hormone (FSH) concentrations, In the present study, we have examined whether the same and similar routes of administration suppresses ovarian function in normally cycling rats and cynomolgus monkeys, Normally cycling female rats received danazol as a single administration either orally, i.m. or s.c. and a separate group also received danazol in silastic capsules, The duration of the dioestrous interval until the next oestrous smear was followed daily and cycle lengths were compared with vehicle-treated groups, Six normally cycling cynomolgus monkeys were followed by daily observation and blood sampling at 2-3 day intervals, After one normal cycle, danazol (200 mg/kg) was administered as a single s.c. injection, Monkeys were followed until the next menses and one cycle thereafter and blood samples were assayed for oestradiol, progesterone and bioactive LH. Oestrous cycle length in vehicle-treated control rats was 4.7 days, A single administration of danazol s.c. at the higher dose prolonged the dioestrous interval to 31.3 days (P