Small interfering RNA targeting CD81 ameliorated arthritis in rats
Small interfering RNA targeting CD81 ameliorated arthritis in rats
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DOI：
10.1016/j.bbrc.2009.06.150
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发表时间：
2009-10-23
影响因子：
3.1
通讯作者：
Nakanishi, Tohru
中科院分区：
文献类型：
--
作者：
Nakagawa, Shuji;Arai, Yuji;Nakanishi, Tohru
CD81属于细胞表面蛋白（四跨膜蛋白）家族，已知为类风湿性关节炎滑膜细胞中的上调元件之一。本研究采用体内电穿孔法检测靶向CD81的小干扰RNA（siCD81）的治疗作用。用siCD81治疗显著改善胶原诱导的关节炎（CIA）大鼠的爪肿胀。在组织学检查中，与对照组和非特异性siRNA组相比，siCD81处理的大鼠的滑膜肥大、骨质侵蚀和关节软骨退变较轻。类风湿调节因子滑膜素的表达被siCD81抑制。因此，通过靶向CD81的治疗性干预可用于治疗类风湿性关节炎。(C)2009 Elsevier Inc. All rights reserved.
CD81 belongs to a family of cell-surface protein ( tetraspanin) known as one of the up-regulated elements in rheumatoid arthritis synoviocytes. In this study, the therapeutic effect of small interfering RNA targeting CD81 (siCD81) was examined by in vivo electroporation method. Treatment with siCD81 significantly ameliorated paw swelling of collagen-induced arthritic ( CIA) rats. In histological examination, hypertrophy of synovium, bone erosion, and degeneration of articular cartilage were milder in rats treated with siCD81 than in the control group and the non-specific siRNA group. Expression of synoviolin, a rheumatoid regulator, was suppressed by siCD81. Thus, therapeutic intervention by targeting CD81 may be used in the treatment of rheumatoid arthritis. (C) 2009 Elsevier Inc. All rights reserved.