PC3 is a cell line characteristic of prostatic small cell carcinoma.
PC3 is a cell line characteristic of prostatic small cell carcinoma.
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DOI：
10.1002/pros.21383
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发表时间：
2011-11
期刊：
影响因子：
2.8
通讯作者：
Huang, Jiaoti
中科院分区：
文献类型：
--
作者：
Tai, Sheng;Sun, Yin;Squires, Jill M.;Zhang, Hong;Oh, William K.;Liang, Chao-Zhao;Huang, Jiaoti
大多数前列腺癌是腺癌，其特征是腺体形成和管腔分化标志物雄激素受体（AR）和前列腺特异性抗原（PSA）的表达。大多数腺癌是惰性的并且依赖于雄激素。抑制 AR 信号传导的激素疗法可以缓解晚期和转移性腺癌患者的症状。前列腺小细胞神经内分泌癌（SCNC）是前列腺癌（PC）的一种变异形式。与腺癌相反，SCNC 的肿瘤细胞不形成腺体，且 AR 和 PSA 呈阴性。 SCNC 极具侵袭性，对激素治疗没有反应。本研究的目的是比较两种最常用的 PC 细胞系 LNCaP 和 PC3 与前列腺腺癌和 SCNC 的重要和相关特征。制备LNCaP和PC3的异种移植肿瘤，并通过免疫组织化学和蛋白质印迹分析与人前列腺腺癌和SCNC比较关键信号分子的表达。 LNCaP 细胞表达 AR 和 PSA，其生长受到雄激素撤退的抑制，类似于人类前列腺腺癌。 PC3细胞不表达AR和PSA，其增殖不依赖于雄激素，与SCNC类似。腺癌细胞和 LNCaP 细胞的神经内分泌标记物和干细胞相关标记物 CD44 呈阴性，而 SCNC 和 PC3 细胞呈阳性。 LNCaP 细胞具有与腺癌相同的细胞角蛋白谱，而 PC3 细胞具有与 SCNC 相似的细胞角蛋白谱。 LNCaP 细胞与腺癌具有共同特征，而 PC3 细胞是 SCNC 的特征。
The majority of the prostatic cancers are adenocarcinomas characterized by glandular formation and the expression of luminal differentiation markers androgen receptor (AR) and prostate-specific antigen (PSA). Most adenocarcinomas are indolent and androgen-dependent. Hormonal therapy that inhibits AR signaling produces symptomatic relief in patients with advanced and metastatic adenocarcinomas. Prostatic small cell neuroendocrine carcinoma (SCNC) is a variant form of prostate cancer (PC). In contrast to adenocarcinoma, the tumor cells of SCNC do not form glands and are negative for AR and PSA. SCNC is extremely aggressive and does not respond to hormonal therapy. The purpose of this study was to compare the important and relevant features of two most commonly used PC cell lines, LNCaP and PC3, with prostatic adenocarcinoma and SCNC. Xenograft tumors of LNCaP and PC3 were prepared and compared with human prostatic adenocarcinoma and SCNC for the expression of key signaling molecules by immunohistochemistry and Western blot analysis. LNCaP cells express AR and PSA and their growth is inhibited by androgen withdrawal, similar to human prostatic adenocarcinoma. PC3 cells do not express AR and PSA and their proliferation is independent of androgen, similar to SCNC. Adenocarcinoma cells and LNCaP cells are negative for neuroendocrine markers and stem cell-associated marker CD44 while SCNC and PC3 cells are positive. LNCaP cells have identical cytokeratin profiles to adenocarcinoma while PC3 cells have cytokeratin profiles similar to SCNC. LNCaP cells share common features with adenocarcinoma while PC3 cells are characteristic of SCNC.
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DOI：
10.1126/science.1189992
发表时间：
2010-07-30
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Goldstein AS;Huang J;Guo C;Garraway IP;Witte ON
通讯作者：
Witte ON
影响因子：
2.1
作者：
Ahlgren, G;Pedersen, K;Abrahamsson, PA
通讯作者：
Abrahamsson, PA
影响因子：
--
作者：
Marchiani, S.;Tamburrino, L.;Baldi, E.
通讯作者：
Baldi, E.
影响因子：
11.2
作者：
Mulholland DJ;Xin L;Morim A;Lawson D;Witte O;Wu H
通讯作者：
Wu H
影响因子：
45.3
作者：
Papandreou, CN;Daliani, DD;Logothetis, CJ
通讯作者：
Logothetis, CJ