Cytokinin regulates the expression of a soybean β-expansin gene by a post-transcriptional mechanism
Cytokinin regulates the expression of a soybean β-expansin gene by a post-transcriptional mechanism
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DOI：
10.1023/a:1005920732211
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发表时间：
1998-06-01
影响因子：
5.1
通讯作者：
Crowell, DN
中科院分区：
文献类型：
--
作者：
Downes, BP;Crowell, DN
除了细胞分裂素饥饿的大豆悬浮培养物外，细胞分裂素诱导的大豆mRNA Cim 1在细胞分裂素作用下积累20-60倍。在这份报告中，我们证明了细胞分裂素诱导的Cim 1 mRNA的稳定性在信息的积累中起着重要的作用。我们还提出的证据表明，细胞分裂素诱导的CIM 1的稳定性被阻断的蛋白磷酸酶抑制剂冈田酸。因此，我们认为，蛋白磷酸酶活性所需的细胞分裂素诱导的稳定性和随后的积累CIM 1在大豆细胞。Cim 1蛋白产物的推导的氨基酸序列与来自各种植物的I组花粉过敏原相似，其构成扩展蛋白的一个亚家族。Cim 1和expansins之间的相关性支持我们的假设，即Cim 1的蛋白质产物定位于细胞壁，并表明Cim 1在细胞分裂素调节的细胞壁扩张中的作用。因此，Cim 1的转录后调节细胞分裂素可能代表细胞分裂素和细胞形状和大小的变化之间的分子联系。
The cytokinin-inducible soybean mRNA Cim1 accumulates 20-60-fold upon cytokinin addition to cytokinin-starved soybean suspension cultures. In this report, we demonstrate that cytokinin-induced stability of the Cim1 mRNA plays an important role in the accumulation of the message. We also present evidence that cytokinin-induced Cim1 stability is blocked by the addition of the protein phosphatase inhibitor okadaic acid. Thus, we suggest that protein phosphatase activity is required for the cytokinin-induced stability and subsequent accumulation of Cim1 in soybean cells. The deduced amino acid sequence of the Cim1 protein product is similar to the group I pollen allergens from various plants, which constitute a subfamily of expansin proteins. The relatedness between Cim1 and the expansins supports our hypothesis that the protein product of Cim1 is localized to the cell wall and suggests a role for Cim1 in cytokinin-regulated cell wall expansion. Thus, post-transcriptional regulation of Cim1 by cytokinin may represent a molecular link between cytokinin and changes in cell shape and size.