Epinephrine Evokes Renalase Secretion via α-Adrenoceptor/NF-κB Pathways in Renal Proximal Tubular Epithelial Cells
Epinephrine Evokes Renalase Secretion via α-Adrenoceptor/NF-κB Pathways in Renal Proximal Tubular Epithelial Cells
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DOI：
10.1159/000355802
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发表时间：
2014-01-01
影响因子：
2.8
通讯作者：
Wang, Niansong
中科院分区：
文献类型：
--
作者：
Wang, Feng;Cai, Hongyan;Wang, Niansong
背景/目的：肾酶是最近发现的一种肾脏特异性单胺氧化酶，可代谢循环中的儿茶酚胺。这些发现为高血压和慢性肾脏疾病提供了新的见解。肾酶主要由近曲小管分泌，可被儿茶酚胺诱导。本研究的目的是研究肾上腺素是否通过α-肾上腺素受体/NF κ B途径诱导肾酶表达。方法：采用肾上腺素刺激HK 2细胞，观察肾上腺素对HK 2细胞肾酶表达的影响。α-肾上腺素能受体拮抗剂酚妥拉明和对甲苯磺酰苯丙氨酰氯甲基酮（TPCK）分别用于阻断α-肾上腺素能受体和敲低转录因子NF κ B B。采用Western blot和定量PCR分析肾酶表达。结果：肾上腺素刺激后，HK 2细胞肾酶蛋白和mRNA水平均升高（P
Background/Aims: Renalase is a recently discovered, kidney-specific monoamine oxidase that metabolizes circulating catecholamines. These findings present new insights into hypertension and chronic kidney diseases. Previous data demonstrated that renalase was mainly secreted from proximal tubules which could be evoked by catecholamines. The purpose of this study is to investigate whether renalase expression is induced by epinephrine via alpha-adrenoceptor/NF kappa B pathways. Methods: HK2 cells were utilized to explore renalase expression in response to epinephrine in vitro. Phentolamine, an alpha-adrenoceptor antagonist, and Tosyl Phenylalanyl Chloromethyl Ketone (TPCK) were used to block alpha-adrenoceptor and to knock down the transcription factor NF kappa B, respectively. Renalase expression was analyzed using Western blot and quantitative PCR. Results: Both protein and mRNA levels of renalase in HK2 cells increased in response to epinephrine (P