VALIDATION OF HEART-RATE AND DOUBLY LABELED WATER AS MEASURES OF METABOLIC-RATE DURING SWIMMING IN CALIFORNIA SEA LIONS
VALIDATION OF HEART-RATE AND DOUBLY LABELED WATER AS MEASURES OF METABOLIC-RATE DURING SWIMMING IN CALIFORNIA SEA LIONS
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DOI：
10.2307/2390559
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发表时间：
1995-04-01
影响因子：
5.2
通讯作者：
WILLIAMS, TM
中科院分区：
文献类型：
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作者：
BOYD, IL;WOAKES, AJ;WILLIAMS, TM
1. 如果我们要充分了解一个物种与其环境之间的相互作用，测量自由放养动物的能量消耗是必不可少的。 本研究探讨了心率（f（H））和双标记水（DLW）作为测量加州海狮（Zalophus californianus）场代谢率（FMR）的有效性. 用直接呼吸法测定了6只幼年海狮24小时的耗氧量和CO2产生量。 呼吸计由水槽上的罩组成，在水槽中，海狮在每个实验中进行15分钟的不同水平的锻炼。 运动方案产生的平均代谢率是预测的基础代谢率（BMR）的2.3倍，平均最大值是预测的BMR的6.27倍。 与直接呼吸测定法同时，使用DLW估计平均CO2产生量，使用f（H）估计O2消耗量，f（H）先前已根据O2消耗量进行校准。 直接呼吸测定法、f（H）和DLW的平均+/- SD O2消耗量分别为11.80 +/- 2.40、11.95 +/- 2.17和15.01 +/- 3.77 ml min-1 kg-1。 配对Student t检验显示，直接呼吸测定法的O2消耗量与DLW和f（H）的估计值之间无显著差异。 DLW测量值范围为直接呼吸测量值的-10%至+86%（平均值+36.4%），f（H）测量值范围为直接呼吸测量值的-28%至+23%（平均值+2.7%）。5. 从f（H）估计的代谢率的范围很大程度上是由于f（H）和O2消耗之间的线性关系的斜率的个体差异。 DLW的代谢率范围可能部分归因于实验持续时间短（24-25 h），但这并不是DLW高估代谢率趋势的原因。 结果表明，DLW和f（H）都是测量加州海狮FMR的有效方法，尽管使用DLW时可能会高估FMR。
1. The measurement of energy expenditures in free-ranging animals is essential if we are to understand fully the interaction between a species and its environment. This study examined the validity of heart rate (f(H)) and doubly labelled water (DLW) as measures of field metabolic rate (FMR) in California Sea Lions (Zalophus californianus).2. Oxygen consumption and CO2 production were measured over 24h by direct respirometry in six juvenile sea lions. The respirometer consisted of a hood over a flume in which the sea lions were exercised to various levels for 15 min periods throughout each experiment. The exercise regime produced a mean metabolic rate which was 2.3 times the predicted basal metabolic rate (BMR) with mean maxima of 6.27 times the predicted BMR.3. Simultaneously with direct respirometry, mean CO2 production was estimated using DLW and O2 consumption was estimated using f(H), which had previously been calibrated against O2 consumption.4. The mean +/- SD O2 consumptions from direct respirometry, f(H) and DLW were 11.80 +/- 2.40, 11.95 +/- 2.17 and 15.01 +/- 3.77 ml min-1 kg-1 respectively. Paired Student's t-tests showed no significant difference between O2 consumption by direct respirometry and the estimates from DLW and f(H). DLW measurements ranged from -10% to +86% of the direct respirometry measurements (mean +36.4%) and f(H) measurements ranged from -28% to +23% of the direct respirometry measurements (mean + 2.7%).5. The range of estimated metabolic rates from f(H) was largely owing to individual differences in the slopes of the linear relationship between f(H) and O2 consumption. The range of metabolic rates from DLW could be partly attributed to the short duration of the experiments (24-25 h) but this was shown not to be the cause of the tendency to overestimate metabolic rate from DLW. It was concluded that both DLW and f(H) are valid methods for measuring FMR in California Sea Lions although it is possible that FMR could be overestimated when using DLW.