Design and performance of an ultra-flexible two-photon microscope for in vivo research
Design and performance of an ultra-flexible two-photon microscope for in vivo research
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DOI：
10.1364/boe.6.004228
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发表时间：
2015-11-01
影响因子：
3.4
通讯作者：
Weber, Bruno
中科院分区：
文献类型：
--
作者：
Mayrhofer, Johannes M.;Haiss, Florent;Weber, Bruno
我们提出了一种高性价比的体内双光子显微镜，具有高度灵活的前端，用于体内研究。我们的设计确保了对感兴趣区域的快速和可重复的访问，包括成像平面的旋转，并最大限度地为动物附近的辅助实验设备提供空间。机械灵活性是实现与大型电动线性阶段，移动物镜在X， Y和Z方向高达130毫米。360度的前端旋转（一个轴的旋转自由）是实现与电动高精度轴承和齿轮的组合。此外，前端的模块化设计基于市售的光学机械部件，可以直接更新未来的扫描技术。该设计超越了以前的移动显微镜设计的移动性，同时保持了高光学性能。(C) 2015美国光学学会
We present a cost-effective in vivo two-photon microscope with a highly flexible frontend for in vivo research. Our design ensures fast and reproducible access to the area of interest, including rotation of imaging plane, and maximizes space for auxiliary experimental equipment in the vicinity of the animal. Mechanical flexibility is achieved with large motorized linear stages that move the objective in the X, Y, and Z directions up to 130 mm. 360 degrees rotation of the frontend (rotational freedom for one axis) is achieved with the combination of a motorized high precision bearing and gearing. Additionally, the modular design of the frontend, based on commercially available optomechanical parts, allows straightforward updates to future scanning technologies. The design exceeds the mobility of previous movable microscope designs while maintaining high optical performance. (C) 2015 Optical Society of America