Evaluation of the aroreira (Schinus terebinthifolius Raddi) in the healing process of surgical incision in the bladder of rats
Evaluation of the aroreira (Schinus terebinthifolius Raddi) in the healing process of surgical incision in the bladder of rats
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DOI：
10.1590/s0102-86502006000800008
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发表时间：
2006-01-01
影响因子：
1.1
通讯作者：
Thiele, Edilson Schwansee
中科院分区：
文献类型：
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作者：
Lucena, Periguari Luiz Holanda de;Ribas Filho, Jurandir Marcondes;Thiele, Edilson Schwansee
目得：评价水乙醇提取物在大鼠膀胱手术切口愈合过程中的实验比较研究。所有患者均行腹部正中切口，行1 cm膀胱切开术，然后用聚乳酸910 5-0缝线进行一次平缝。然后将动物分为两组，每组20只，并命名为aroeira组（GA）和对照组（GC）。在第一组中，将80%水醇植物提取物以100 mg/kg的剂量一次性注入腹腔。第二组，注射0.9%的生理盐水溶液代替芦荟提取物。每组分为两个亚组（SGA和SGC），每组10只动物。根据其计划死亡时间，将术后第3天处死的动物命名为SGA 3和SGC 3亚组，将术后第7天处死的动物命名为SGA 7和SGC 7亚组。结果：手术切口经苏木精-伊红染色和Masson三色染色后，膀胱组织病理学检查均为阴性。宏观分析显示，SGC组3只大鼠和SGA组1只大鼠的手术切口发生感染，26只大鼠（9只属于SGC组，17只属于SGA组）发生腹膜粘连。显微镜评价显示，在第3天（p=0.045）和第7天（0=0.002），SGC中的急性炎症过程更严重。在SGA中，可以看到更显著的胶原化（p=0.001），至于第3天的血管新生证据（p=0.002）。慢性炎症反应（p=0.006）和更强烈的血管新生（p=0.001），观察到在第7天。结论：水醇沉香提取物在大鼠膀胱切开术的愈合过程中有良好的效果。
PURPOSE: To evaluate the hydroalcoholic aroeira extract in the healing process of surgical incisions in the bladder of rats in an experimental comparative study.METHODS: Forty male Wistar rats were used. All of them underwent a median abdominal incision, with an 1cm cystotomy, followed by one plain suture with separated stitches of poliglactine 910 5-0. The animals were then divided into two groups of 20 rats each, and named the aroeira group (GA) and the control group (GC). In the first one, 80% hydro-alcoholic plant extract was injected in the peritoneal cavity, in a one dose of 100mg/kg. The second group, a 0.9% saline solution was injected in place of aroeira extract. Each group was divided into two subgroups (SGA and SGC) of 10 animals. According to their scheduled death, they were named subgroups SGA3 and SGC3 when killed in the 3th day after surgery and subgroups SGA7 and SGC7 in the 7th day. The abdominal cavity and the bladder suture were macroscopically evaluated.RESULTS: The microscopic analysis of the surgical incision in the bladder was performed by means of the hematoxilin-eosine stain and the trichrome of Masson. The macroscopic analysis showed an infection in the surgical incision in three rats of the SGC group and in one of the SGA, and peritoneal adhesion in the 26 rats belonging nine to SGC and 17 in the SGA. The microscopic evaluation revealed a more severe acute inflammation process in the SGC on the 3th (p=0.045) and in the 7th (0=0.002) days. In the SGA, a more significant collagenization (p=0.001) could be seen, as far as the evidence of vascular neoformation (p=0.002) on the 3rd day. Chronic inflammatory reaction (p=0.006) and a more intense vascular neoformation (p=0.001) were observed in the 7th day.CONCLUSION: The hydroalcoholic aroeira extract had a favorable effect in the healing process of cystotomies done in rats.