Establishment and Characterization of a Newly Established Diabetic Gerbil Line.
Establishment and Characterization of a Newly Established Diabetic Gerbil Line.
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新建立的糖尿病沙鼠系的建立和表征。
DOI：
10.1371/journal.pone.0159420
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Chen Z
中科院分区：
文献类型：
--
作者：
Li X;Lu J;Wang Y;Huo X;Li Z;Zhang S;Li C;Guo M;Du X;Chen Z
当近交沙鼠的一个亚系最近发现血糖水平升高时，我们的目的是选择性地培育自发性糖尿病沙鼠。然后我们研究了血清胰岛素、甘油三酯、胆固醇、瘦素、脂联素等特征，并探讨了糖尿病表型的潜在分子机制。通过监测每只动物的血糖，选择性地近亲繁殖沙鼠的自发糖尿病系。使用 ELISA 试剂盒检测血清胰岛素、脂联素和瘦素水平。通过蛋白质印迹和实时定量 PCR (qPCR) 检测肝脏、骨骼肌和白色脂肪中 GLUT4、Akt、瘦素、脂联素和钙蛋白酶 10 (CAPN10) 的表达水平。结果表明，从F0（21.33%）到F6（38.46%），随着繁殖代数的增加，FPG≥5.2（mmol/l）、PG2h≥6.8（mmol/l）以及FPG≥5.2和PG2h≥6.8（mmol/l）的动物比例增加。这些糖尿病沙鼠表现出胰岛素抵抗和瘦素抵抗以及血清中脂联素水平降低。我们还分别观察到骨骼肌中脂联素表达减少和瘦素表达增加。这些结果表明我们已经初步建立了自发的糖尿病沙鼠系，并且糖尿病表型可能是通过瘦素和脂联素表达的改变来解释的。
We aimed to selectively breed a spontaneous diabetic gerbil when a sub-line of inbred gerbil showed increased blood glucose levels was found recently. Then we investigated the characteristics including the serum insulin, triglyceride, cholesterol, leptin, adiponectin and explored the underlying molecular mechanism for the diabetic phenotype. The spontaneous diabetic line of gerbils was selectively inbreed the sub-line of gerbil by monitoring blood glucose of each animal. The serum insulin, adiponectin, and leptin levels were tested using an ELISA kit. The expression levels of GLUT4, Akt, leptin, adiponectin, and calpain 10 (CAPN10) were tested by western blot and Quantitative Real-time PCR (qPCR) in liver, skeletal muscle, and white adipose. Our results show that the percentages of animals with FPG≥5.2 (mmol/l), PG2h≥6.8 (mmol/l) and both FPG≥5.2 and PG2h≥6.8 (mmol/l) were increased with the number of breeding generations from F0 (21.33%) to F6 (38.46%). These diabetic gerbils exhibited insulin resistance and leptin resistance as well as decreased adiponectin level in the serum. We also observed decreased expression of adiponectin and increased expression of leptin in the skeletal muscle, respectively. These results indicate that we have primarily established a spontaneous diabetic gerbil line, and the diabetic phenotypes may have been accounted for by altered expression of leptin and adiponectin.