Identification of acetic acid bacteria isolated from fruits collected in Thailand.
Identification of acetic acid bacteria isolated from fruits collected in Thailand.
复制标题
从泰国采集的水果中分离出的醋酸菌的鉴定。
DOI：
10.2323/jgam.50.47
复制
发表时间：
2004
期刊：
影响因子：
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通讯作者：
Yuzo Yamada
中科院分区：
文献类型：
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作者：
Apisit Seearunruangchai;S. Tanasupawat;S. Keeratipibul;C. Thawai;T. Itoh;Yuzo Yamada
分为六个属：Acetobacter Beijerinck 1898，Gluconacetobacter Asai 1935，Acetobacter Urakami等1989，Gluconacetobacter Yamada等1997，Asaia Yamada等2000，和Kozakia Lisdiyanti等2002（De Ley等，1984; Gillis和De Ley，1980; Lisdiyanti等人，2002年; Stackebrandt等人，1988; Uhlig等人，1986; Urakami等人，1989; Yamada等人，1997年，2000年）。已经从酒精饮料、醋、水果、花、蜂蜜、蜜蜂、甘蔗汁、土壤和水中分离出乙酸细菌（Saeki等人，1997; Swings，1992; Yamada等人，1999年）。最近，基于DNA碱基组成、DNA-DNA相似性和16 S rRNA基因测序，描述了许多种乙酸细菌，尤其是乙酸杆菌属（Acetobacter）（Cleenwerck等人，2002; Lisdiyanti等人，2000，2001; Sokollek等人，1998年）。由于泰国位于热带地区，我们预计在这个国家会发现许多种醋酸菌。本研究旨在从泰国采集的水果和其他源材料中分离和鉴定醋酸菌。从泰国曼谷和一些省份收集的多种水果和其他源材料中，通过富集培养方法分离乙酸细菌。将收集的源材料在由2.0%D-葡萄糖、5.0%乙醇和1.0%酵母提取物组成的GEY液体培养基（15 ml）中在pH 4.5和30°C下孵育3-5天。泰国水果中醋酸菌的分离鉴定
lowing six genera: Acetobacter Beijerinck 1898, Gluconobacter Asai 1935, Acidomonas Urakami et al. 1989, Gluconacetobacter Yamada et al. 1997, Asaia Yamada et al. 2000, and Kozakia Lisdiyanti et al. 2002 (De Ley et al., 1984; Gillis and De Ley, 1980; Lisdiyanti et al., 2002; Stackebrandt et al., 1988; Uhlig et al., 1986; Urakami et al., 1989; Yamada et al., 1997, 2000). The acetic acid bacteria have been isolated from alcoholic beverages, vinegar, fruits, flowers, honey, bees, sugar cane juices, soil, and water (Saeki et al., 1997; Swings, 1992; Yamada et al., 1999). Recently, many species of acetic acid bacteria were described, especially in the genus Acetobacter on the basis of DNA base composition, DNA-DNA similarity, and 16S rRNA gene sequencing (Cleenwerck et al., 2002; Lisdiyanti et al., 2000, 2001; Sokollek et al., 1998). Since Thailand is located in a tropical area, we have expected that many species of acetic acid bacteria will be found in this country. This study aims at the isolation and identification of acetic acid bacteria from fruits and other source materials collected in Thailand. Acetic acid bacteria were isolated by an enrichment culture approach from many kinds of fruits and other source materials collected in Bangkok and in some provinces of Thailand. The collected source materials were incubated at pH 4.5 and 30°C for 3–5 days in a GEY liquid medium (15 ml) composed of 2.0% D-glucose, 5.0% ethanol, and 1.0% yeast extract. When miIdentification of acetic acid bacteria isolated from fruits collected in Thailand