Human cortical neuronal cell line: establishment from a patient with unilateral megalencephaly.
Human cortical neuronal cell line: establishment from a patient with unilateral megalencephaly.
复制标题
人皮质神经元细胞系：从单侧巨脑畸形患者建立。
DOI：
10.1126/science.1692158
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Snyder,SH
中科院分区：
文献类型：
--
作者：
Ronnett,GV;Hester,LD;Nye,JS;Connors,K;Snyder,SH
从单侧巨脑畸形患者（一种与未成熟神经元细胞持续增殖相关的疾病）身上获得的人类大脑皮质神经元连续培养中建立了细胞系。当在神经生长因子、1-异丁基-3-甲基黄嘌呤和3 ',5 ' -单磷酸二丁基腺苷（cAMP）存在下分化时，细胞表现出成熟的神经元形态，具有许多长而广泛的分枝突起，具有棘和曲张。神经丝蛋白和神经元特异性烯醇化酶（选择性神经元标记物）染色呈阳性，但胶质标记物（如胶质纤维酸性蛋白、S-100和髓鞘碱性蛋白）染色呈阴性。细胞对神经递质γ-氨基丁酸（GABA）、谷氨酸、生长抑素、胆囊收缩素-8和血管活性肠多肽也呈阳性染色。这些细胞可能有助于人类中枢神经系统神经元的表征。
A cell line has been established in continuous culture of human cerebral cortical neurons obtained from a patient with unilateral megalencephaly, a disorder associated with continued proliferation of immature neuronal cells. When differentiated in the presence of nerve growth factor, 1-isobutyl-3-methylxanthine, and dibutyryl adenosine 3′,5′-monophosphate (cAMP), the cells display mature neuronal morphology with numerous long, extensively branched processes with spines and varicosities. The cells stain positively for neurofilament protein and neuron-specific enolase (selective neuronal markers) but are negative for glial markers, such as glial fibrillary acidic protein, S-100, and myelin basic protein. The cells also stain positively for the neurotransmitters γ-aminobutyric acid (GABA), glutamate, somatostatin, cholecystokinin-8, and vasoactive intestinal polypeptide. These cells may facilitate characterization of neurons in the human central nervous system.