Phytochemical characterization of Tabernanthe iboga root bark and its effects on dysfunctional metabolism and cognitive performance in high-fat-fed C57BL/6J mice.
Phytochemical characterization of Tabernanthe iboga root bark and its effects on dysfunctional metabolism and cognitive performance in high-fat-fed C57BL/6J mice.
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DOI：
10.31665/jfb.2018.3154
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发表时间：
2018-09-01
期刊：
影响因子：
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通讯作者：
Stevens, Jan F
中科院分区：
文献类型：
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作者：
Bading-Taika, Bayissi;Akinyeke, Tunde;Stevens, Jan F
Tabernanthe iboga的根皮制剂长期以来一直用于中非和西非的传统医学，以对抗疲劳，在仪式中作为神经兴奋剂，并用于治疗糖尿病。T. iboga，iboclavia，因其为吸毒成瘾者对类阿片的渴望提供救济而引起世界许多国家的关注。利用植物代谢组学的方法，我们检测到5种酚类化合物，包括3-O-咖啡酰奎宁酸，和30种生物碱，其中7种是以前报道的T。伊波加根皮在报道iboga提取物含有促胰岛素剂之后，我们的目的是确定iboga根皮的水提取物对高脂饮食（HFD）诱导的高血糖症的潜在缓解作用以及其对雄性C57 BL/6 J小鼠认知功能的影响。与喂食低脂饮食（LFD）的小鼠相比，喂食HFD 10周的小鼠产生代谢综合征的表现，例如体重增加和葡萄糖、三酰甘油、总胆固醇、LDL-胆固醇、胰岛素、瘦素和促炎介质（IL-6、MCP-1、ICAM-1）的血浆水平增加。以0.83（低）和2.07（高）mg/kg/天的iboga提取物剂量补充HFD并没有改善这些HFD诱导的代谢效应，除了低剂量组中血浆MCP-1的减少，表明抗炎作用。当HFD小鼠在水迷宫中进行测试时，与仅接受HFD的小鼠相比，高剂量的伊博加提取物引起了空间学习和记忆的海马依赖性损伤。
Preparations of the root bark of Tabernanthe iboga have long been used in Central and West African traditional medicine to combat fatigue, as a neuro-stimulant in rituals, and for treatment of diabetes. The principal alkaloid of T. iboga, ibogaine, has attracted attention in many countries around the world for providing relief for opioid craving in drug addicts. Using a plant metabolomics approach, we detected five phenolic compounds, including 3-O-caffeoylquinic acid, and 30 alkaloids, seven of which were previously reported from T. iboga root bark. Following a report that iboga extracts contain insulinotropic agents, we aimed to determine the potential alleviating effects of the water extract of iboga root bark on high-fat diet (HFD)-induced hyperglycemia as well as its effects on cognitive function in male C57BL/6J mice. Feeding a HFD to mice for 10 weeks produced manifestations of metabolic syndrome such as increased body weight and increased plasma levels of glucose, triacylglycerols, total cholesterol, LDL-cholesterol, insulin, leptin, and pro-inflammatory mediators (IL-6, MCP-1, ICAM-1), as compared to mice fed a low-fat diet (LFD). Supplementation of HFD with iboga extract at ibogaine doses of 0.83 (low) and 2.07 (high) mg/kg/day did not improve these HFD-induced metabolic effects except for a reduction of plasma MCP-1 in the low dose group, indicative of an anti-inflammatory effect. When the HFD mice were tested in the water maze, the high-dose iboga extract caused hippocampus-dependent impairments in spatial learning and memory, as compared to mice receiving only a HFD.