NEXT-GENERATION SEQUENCING AND GENOME EVOLUTION IN ALLOPOLYPLOIDS
NEXT-GENERATION SEQUENCING AND GENOME EVOLUTION IN ALLOPOLYPLOIDS
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DOI：
10.3732/ajb.1100395
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发表时间：
2012-02-01
影响因子：
3
通讯作者：
Soltis, Douglas E.
中科院分区：
文献类型：
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作者：
Buggs, Richard J. A.;Renny-Byfield, Simon;Soltis, Douglas E.
本研究的目的：杂交和多倍化（异源多倍体）在植物进化中普遍存在，但追踪异源多倍体组成基因组的起源和随后的进化一直具有挑战性。基因组加倍极大地使遗传分析复杂化，这长期阻碍了研究，因为大多数异源多倍体物种是“非模式”生物。然而，在测序和基因组学技术的最新进展，现在提供了前所未有的机会，分析许多遗传标记在多个人在任何organis.Methods：在这里，我们回顾了下一代测序技术的应用研究异源多倍体基因组进化的三个方面：在两个最近形成的婆罗门参异源多倍体中重复的基因丢失和表达，Tragopogon miscellus中的基因组间相互作用和染色体进化，以及烟草异源多倍体中的重复DNA进化。第一次，我们可以在基因组的尺度上探索自然异源多倍体中正在进行的进化过程，而不局限于研究充分的作物和遗传模型。这些方法可以很容易和廉价地应用于许多其他植物物种，使任何进化挑衅系统一个新的“模型”系统。
Premise of the study: Hybridization and polyploidization (allopolyploidy) are ubiquitous in the evolution of plants, but tracing the origins and subsequent evolution of the constituent genomes of allopolyploids has been challenging. Genome doubling greatly complicates genetic analyses, and this has long hindered investigation in that most allopolyploid species are "nonmodel" organisms. However, recent advances in sequencing and genomics technologies now provide unprecedented opportunities to analyze numerous genetic markers in multiple individuals in any organism.Methods: Here we review the application of next-generation sequencing technologies to the study of three aspects of allopolyploid genome evolution: duplicated gene loss and expression in two recently formed Tragopogon allopolyploids, intergenomic interactions and chromosomal evolution in Tragopogon miscellus, and repetitive DNA evolution in Nicotiana allopolyploids.Key results: For the first time, we can explore on a genomic scale the evolutionary processes that are ongoing in natural allopolyploids and not be restricted to well-studied crops and genetic models.Conclusions: These approaches can be easily and inexpensively applied to many other plant species making any evolutionarily provocative system a new "model" system.