Fluorescence resonance energy transfer between NaYF4:Yb,Tm upconversion nanoparticles and gold nanorods: Near-infrared responsive biosensor for streptavidin
Fluorescence resonance energy transfer between NaYF4:Yb,Tm upconversion nanoparticles and gold nanorods: Near-infrared responsive biosensor for streptavidin
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DOI：
10.1016/j.jlumin.2013.11.052
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发表时间：
2014-03
影响因子：
3.6
通讯作者：
Shuang Zhang;Jing Wang;Wen Xu;Boting Chen;Wei Yu;Lin Xu;Hongwei Song
中科院分区：
文献类型：
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作者：
Shuang Zhang;Jing Wang;Wen Xu;Boting Chen;Wei Yu;Lin Xu;Hongwei Song
我们提出了一个荧光共振能量转移（FRET）系统，使用上转换纳米粒子（UCNPs）和金纳米棒（GNRs）作为能量供体-受体对直接测定链霉亲和素在近红外（NIR）区域。NaYF 4：Yb，TmUCNPs在980 nm激发下，在800 nm处有较强的发光，被用作能量供体。选择在800 nm附近表现出强表面等离子体吸收的GNR作为受体以淬灭UCNP的800 nm发射。供体纳米粒子（UCNPs）的发射光谱与受体纳米粒子（GNRs）的吸收光谱存在重叠。然后使用该基于UCNP的FRET系统来确定链霉亲和素的量。在该系统中，NaYF 4：Yb，Tm UCNPs与生物素缀合，而GNRs与链霉亲和素缀合。当将GNRs添加到UCNPs中时，链霉亲和素优选与生物素结合，并减少供体和受体NPs之间的间距。因此，发生FRET，并且获得发光猝灭效率与链霉亲和素浓度之间的线性关系。由于UCNPs作为能量供体的上述优点和GNRs的强猝灭能力，获得了令人满意的分析性能。
We represent a fluorescence resonance energy transfer (FRET) system using upconversion nanoparticles (UCNPs) and the gold nanorods (GNRs) as the energy donor–acceptor pair for directly determining streptavidin in near-infrared (NIR) region. NaYF4:Yb,Tm UCNPs, which had a strong emission at 800 nm under 980-nm excitation, were adopted as the energy donor. The GNRs, which demonstrated strong surface plasmon absorption around 800 nm, were chosen as acceptor to quench the 800 nm emissions of the UCNPs. There had the spectral overlap between the emission of the donor nanoparticles (UCNPs) and the absorption of the acceptor nanoparticles (GNRs). This UCNP-based FRET system was then used to determine the amount of streptavidin. In this system, NaYF4:Yb,Tm UCNPs conjugated with biotin, while GNRs conjugated with streptavidin. When added GNRs into UCNPs, the streptavidin were preferred to bind with biotin and decreased spacing between the donor and acceptor NPs. Consequently, FRET occurred and a linear relationship between the luminescence quenching efficiency and the concentration of streptavidin was obtained. Owing to the aforementioned merits of UCNPs as an energy donor and the strong quenching ability of GNRs, satisfactory analytical performances have been acquired.