Microstructuring of polymer films for sensitive genotyping by real-time PCR on a centrifugal microfluidic platform
Microstructuring of polymer films for sensitive genotyping by real-time PCR on a centrifugal microfluidic platform
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DOI：
10.1039/c004954a
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发表时间：
2010-01-01
期刊：
影响因子：
6.1
通讯作者：
von Stetten, Felix
中科院分区：
文献类型：
--
作者：
Focke, Maximilian;Stumpf, Fabian;von Stetten, Felix
我们提出了一种新的工艺流程，使原型的微流控盒制成的聚合物薄膜。通过对22份DNA样本进行微流控基因分型检测，包括耐甲氧西林金黄色葡萄球菌（MRSA）感染患者的临床分离株，证明了其高性能。微流控盒（磁盘）制造的一种新的工艺称为微热成型软光刻（μ TSL）。与传统的微热成型相比，采用正模可以实现更高的成型精度和非常容易的脱模。高复制精度与几何磁盘到磁盘的变化小于1%是典型的。我们描述和描述了壁厚微流控盒的制造和应用
We present a novel process flow enabling prototyping of microfluidic cartridges made out of polymer films. Its high performance is proven by implementation of a microfluidic genotyping assay testing 22 DNA samples including clinical isolates from patients infected by methicilin-resistant Staphylococcus aureus (MRSA). The microfluidic cartridges (disks) are fabricated by a novel process called microthermoforming by soft lithography (mu TSL). Positive moulds are applied allowing for higher moulding precision and very easy demoulding when compared to conventional microthermoforming. High replication accuracies with geometric disk-to-disk variations of less than 1% are typical. We describe and characterise fabrication and application of microfluidic cartridges with wall thicknesses