High-throughput relative DPPH radical scavenging capacity assay
High-throughput relative DPPH radical scavenging capacity assay
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DOI：
10.1021/jf0611668
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发表时间：
2006-10-04
影响因子：
6.1
通讯作者：
Yu, Liangli (Lucy)
中科院分区：
文献类型：
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作者：
Cheng, Zhihong;Moore, Jeffrey;Yu, Liangli (Lucy)
本研究建立了一种高通量的相对DPPH自由基清除能力（RDSC）测定方法。该RDSC试验易于进行，具有可接受的准确度（90-110%回收率）、精密度[3.9-7.0%合并相对标准偏差（RSD）]和重现性（2.2%和3.5%日间和日内RSD）。该测定报告了抗氧化剂样品的RDSC值，这使得可以比较在不同实验室测定的抗氧化剂的DPPH自由基清除能力。对于亲水性抗氧化剂，RDSC测定可以在含水醇和丙酮中进行，或者对于亲脂性抗氧化剂，在没有增溶剂的有机溶剂中进行，这使得可以直接比较亲水性和亲脂性抗氧化剂的自由基清除能力。此外，高通量RDSC测定可用于EC 50值的估计。高通量RDSC分析可用于筛选和研究潜在的天然抗氧化剂。
A high-throughput relative 2,2-diphenyl-1-picryhydrazyl (DPPH) radical scavenging capacity (RDSC) assay was developed and validated in the present study. This RDSC assay is easy to perform and has acceptable accuracy (90-110% recovery), precision [3.9-7.0% pooled relative standard deviation (RSD)], and reproducibility (2.2 and 3.5% interday and intraday RSD). This assay reports the RDSC values for antioxidant samples, which make it possible to compare the DPPH radical scavenging capacities of antioxidants determined in different laboratories. The RDSC assay may be conducted in aqueous alcohol and acetone for hydrophilic antioxidants or in the organic solvents for lipophilic antioxidants without solubilizing agents, which makes it possible to directly compare the radical scavenging capacities of hydrophilic and lipophilic antioxidants. In addition, the high-throughput RDSC assay could be utilized for EC50 value estimation. The high-throughput RDSC assay may be used for screening and investigating potential natural antioxidants.