The stress-induced MAP kinase p38 regulates endocytic trafficking via the GDI:Rab5 complex
The stress-induced MAP kinase p38 regulates endocytic trafficking via the GDI:Rab5 complex
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DOI：
10.1016/s1097-2765(01)00189-7
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发表时间：
2001-02-01
期刊：
影响因子：
16
通讯作者：
Gruenberg, J
中科院分区：
文献类型：
--
作者：
Cavalli, V;Vilbois, F;Gruenberg, J
早期的胞内膜运输是由小的GTP酶Rab5调节的，它在GTP和GDP结合的状态之间以及在膜和胞浆之间循环。后一个循环依赖于GDI，它在细胞质的水环境中作为RAB载体发挥作用。在这里，我们报告了GDI：Rab5复合体的形成是由我们纯化的胞浆因子刺激的，然后鉴定为p38MAPK。我们发现，p38在Rab5的胞浆周期中调节GDI，并调节体内的内吞作用。我们的观察揭示了内吞作用和p38依赖的应激反应之间存在串扰，从而提供了内吞作用可以受环境调节的分子证据。
Early endocytic membrane traffic is regulated by the small GTPase Rab5, which cycles between GTP- and GDP-bound states as well as between membrane and cytosol. The latter cycle depends on GDI, which functions as a Rab vehicle in the aqueous environment of the cytosol. Here, we report that formation of the GDI:Rab5 complex is stimulated by a cytosolic factor that we purified and then identified as p38 MAPK. We find that p38 regulates GDI in the cytosolic cycle of Rab5 and modulates endocytosis in vivo. Our observations reveal the existence of a cross-talk between endocytosis and the p38-dependent stress response, thus providing molecular evidence that endocytosis can be regulated by the environment.