Involvement of template-activating factor I/SET in transcription of adenovirus early genes as a positive-acting factor
Involvement of template-activating factor I/SET in transcription of adenovirus early genes as a positive-acting factor
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DOI：
10.1128/jvi.80.2.794-801.2006
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发表时间：
2006-01-01
影响因子：
5.4
通讯作者：
Nagata, K
中科院分区：
文献类型：
--
作者：
Haruki, H;Okuwaki, M;Nagata, K
与病毒核心蛋白VII复合的腺病毒基因组（腺病毒DNA-蛋白VII复合物）至少是腺病毒早期基因转录的真正模板。据信，高碱性蛋白 VII 与细胞组蛋白一样，是基因组功能的负调节因子。使用腺病毒 DNA-蛋白 VII 复合物对体外复制和转录系统进行的分析表明，复合物的重塑对于有效的 DNA 复制和转录至关重要。我们确定了宿主酸性蛋白、模板激活因子 I (TAF-I)、TAF-II 和 TAF-III 作为腺病毒 DNA-蛋白 VII 复合物复制的刺激因子。最近，据报道，腺病毒DNA与TAF-I和另一种宿主酸性蛋白pp32相互作用(Y.Xue，J.S.Johnson，D.A.Ornelles，J.Lieberman，and D.A.Engel，J.Virol.79：2474-2483，2005)。我们发现，在感染的早期阶段，TAF-I 与腺病毒感染细胞中的蛋白 VII 相互作用并共定位，但 pp32 则不然。尽管pp32具有与蛋白VII相互作用的潜在能力，但pp32在体外并不能重塑腺病毒DNA-蛋白VII复合物。小干扰RNA介导的TAF-I表达敲低导致早期基因转录时间的延迟。这些结果提供了TAF-I在腺病毒感染周期的早期阶段发挥重要作用的证据。
The adenovirus genome complexed with viral core protein VII (adenovirus DNA-protein VII complex) at least is the bona fide template for transcription of adenovirus early genes. It is believed that the highly basic protein VII, like cellular histones, is a negative regulator for genome functions. Analyses with in vitro replication and transcription systems using the adenovirus DNA-protein VII complex have revealed that remodeling of the complex is crucial for efficient DNA replication and transcription. We identified host acidic proteins, template-activating factor I (TAF-I), TAF-II, and TAF-III as stimulatory factors for replication from the adenovirus DNA-protein VII complex. Recently, it was reported that the adenovirus DNA interacts with TAF-I and pp32, another host acidic protein (Y. Xue, J. S. Johnson, D. A. Ornelles, J. Lieberman, and D. A. Engel, J. Virol. 79:2474-2483, 2005). We found that TAF-I interacts and colocalizes with protein VII in adenovirus-infected cells during the early phases of infection, but pp32 does not. Although pp32 had the potential ability to interact with protein VII, pp32 did not remodel the adenovirus DNA-protein VII complex in vitro. Small interfering RNA-mediated knockdown of TAF-I expression leads to the delay of the transcription timing of early genes. These results provide evidence that TAF-I plays an important role in the early stages of the adenovirus infection cycle.