CpG oligodeoxynucleotide and montanide ISA 206 adjuvant combination augments the immune responses of a recombinant FMDV vaccine in cattle
CpG oligodeoxynucleotide and montanide ISA 206 adjuvant combination augments the immune responses of a recombinant FMDV vaccine in cattle
复制标题
DOI：
10.1016/j.vaccine.2011.08.072
复制
发表时间：
2011-10-19
期刊：
影响因子：
5.5
通讯作者：
Yu, Yongli
中科院分区：
文献类型：
--
作者：
Ren, Jiling;Yang, Liang;Yu, Yongli
口蹄疫(FMD)是偶蹄动物的一种高度传染性疾病。为了防止口蹄疫病毒的传播，在发展中国家使用灭活病毒疫苗为动物接种疫苗。然而，也存在安全方面的担忧。此外，很难区分接种疫苗的动物和自然感染的动物。在我们的实验室，我们已经开发出一种名为A7的重组口蹄疫疫苗。A7含有多个B细胞和T细胞表位，存在于口蹄疫病毒衣壳蛋白(VP1)中。为提高A7疫苗的免疫原性，将CpG ODN RW03与Montanide ISA 206(ISA)联合制成疫苗(A7+ISA+CpG ODN RW03)免疫小鼠和牛。结果表明，CpG ODN RW03与ISA联合使用可促进A7在小鼠和牛体内诱导强烈而持久的特异性抗体应答。口蹄疫病毒攻毒后，A7+ISA+CpG ODN RW03免疫组的保护率为80%(4/5)，优于A7+ISA组(25%，1/4)和灭活疫苗免疫组(50%，2/4)。这些结果表明，CpG ODN RW03可以与Montanide ISA 206一起用作牛重组口蹄疫病毒的有效佐剂。(C)2011爱思唯尔有限公司。保留所有权利。
Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals. To prevent the spread of FMDV, inactivated virus vaccines are used to immunize animals in developing countries. However, there are safety concerns. In addition, it is difficult to distinguish the vaccinated animals from those naturally infected ones. In our lab, we have developed a recombinant FMDV vaccine named A7. A7 contained multiple B cell and T cell epitopes, which reside in a capsid protein (VP1) of FMDV. To enhance its immunogenicity, A7 was formulated with CpG ODN RW03 in combination with Montanide ISA 206 (ISA), and the resultant vaccine (A7 + ISA + CpG ODN RW03) was used to immunize mice and cattle. It was found that CpG ODN RW03 and ISA combination could facilitate A7 to induce a vigorous and long-lasting specific antibody response in mice and cattle. After FMDV challenge, 80% (4/5) of the calves immunized with A7 + ISA + CpG ODN RW03 were protected, which was superior to those immunized with A7 + ISA (25%, 1/4) or inactivated FMDV vaccine (50%, 2/4). These findings suggest that CpG ODN RW03 could be used with Montanide ISA 206 as a potent adjuvant for recombinant FMDV in cattle. (C) 2011 Elsevier Ltd. All rights reserved.