A continuous culture biofilm model of cariogenic responses
A continuous culture biofilm model of cariogenic responses
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DOI：
10.1046/j.1365-2672.2001.01263.x
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发表时间：
2001-03-01
影响因子：
4
通讯作者：
Allison, C
中科院分区：
文献类型：
--
作者：
Hodgson, RJ;Lynch, RJM;Allison, C
目的：验证一个体外模型，用于分析细菌群体对糖挑战的生理和生态反应，以及随后牙釉质矿化的变化。方法和结果：在连续培养系统中，一个由7种生物组成的细菌联合体以生物膜模式生长在牙釉质和羟基磷灰石(HA)表面，并暴露在反复的蔗糖挑战中。这在系统内产生了“pH循环”的条件。对羟基磷灰石表面的菌落进行了计数。观察了不同处理之间不同种群的相对比例和总活菌数的变化。釉质切片的显微放射照片显示，随着蔗糖浓度的增加，脱矿率增加。形成的病变与活体中发现的白斑病变相似。激光共聚焦扫描显微镜观察到生物膜形成的质量差异。结论：体外模型已被验证用于分析细菌种群对蔗糖挑战的生理和生态响应，以及随后的釉质矿化变化。研究的意义和影响：该模型有助于研究应用可能的防龋剂和伴随的釉质矿化变化的细菌种群的变化。
Aims: To validate an in vitro model for the analysis of physiological and ecological responses to sugar challenge in bacterial populations, and subsequent changes in enamel mineralization.Methods and Results: A seven-organism bacterial consortium was grown in a biofilm mode on enamel and hydroxyapatite (HA) surfaces in a continuous culture system and exposed to repeated sucrose challenges. This produced 'pH-cycling' conditions within the system. Populations on HA surfaces were enumerated. Changes in relative proportions of the different populations, and in the total viable count, were observed, between different treatments. Microradiography of the enamel sections showed increasing demineralization with increasing sucrose concentration. The lesions formed were similar to 'white-spot' lesions found in vivo. Differences in the quality of biofilms formed were also observed using Confocal Laser Scanning Microscopy.Conclusions: An in vitro model has been validated for the analysis of both physiological and ecological responses to sucrose challenges in bacterial populations, and subsequent changes in enamel mineralization.Significance and Impact of the Study: This model should facilitate the study of changes in bacterial populations in response to application of putative anticaries agents and concomitant changes in enamel mineralization.