Proteome analysis by two-dimensional gel electrophoresis and mass spectrometry: strengths and limitations
Proteome analysis by two-dimensional gel electrophoresis and mass spectrometry: strengths and limitations
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DOI：
10.1016/s0165-9936(03)00508-9
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发表时间：
2003-05-01
影响因子：
13.1
通讯作者：
Beranova-Giorgianni, S
中科院分区：
文献类型：
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作者：
Beranova-Giorgianni, S
双向凝胶电泳、质谱和生物信息学是当前蛋白质组学技术的关键组成部分。本文综述了基于2DE的蛋白质组学方法的基本概念，讨论了该方法的优点和局限性，并重点介绍了基于2DE技术的一些最新进展。(C)出版社：Elsevier Science B.V.
Two-dimensional gel electrophoresis, mass spectrometry and bioinformatics are the key components of current proteomics technology. The purpose of this review is to give an overview of the basic concepts of 2DE-based proteomics methodology, to discuss the advantages and limitations of the approach, and to highlight some recent advancements in 2DE-based technology. (C) 2003 Published by Elsevier Science B.V.