The timecourse of neuronal connections of the rotundoectostriatal pathway in chicks (Gallus gallus) during embryogenesis: a retrograde transport study.
The timecourse of neuronal connections of the rotundoectostriatal pathway in chicks (Gallus gallus) during embryogenesis: a retrograde transport study.
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胚胎发生过程中小鸡（原鸡）圆形外纹状体通路神经元连接的时间进程：一项逆行运输研究。
DOI：
10.1017/s0952523800176096
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发表时间：
2000
影响因子：
1.9
通讯作者：
Karten,HJ
中科院分区：
文献类型：
--
作者：
Wu,CC;Charlton,RK;Karten,HJ
鸟类离视网膜网途径被认为与哺乳动物离视网膜网途径是同源的。圆核向外纹状体的投射相当于哺乳动物的枕核向外纹状体皮质的投射。在这个系统中，视顶盖将视网膜输入传递到圆核，然后圆核上升到端脑的外纹状体。鉴于鸡视网膜离体系统在发育过程中成熟早，本研究试图探讨胚胎圆外纹状体通路的神经元连接的时间过程。我们用多次注射霍乱毒素B亚单位（CT B）在鸡胚的外纹状体逆行追踪投射到圆核。我们发现CTb标记的神经元圆核在胚胎7.5-8天。到胚胎第8-8.5天，在圆核中看到CTb标记的神经元数量增加。注意到，圆核和外纹状体之间的这种初始连接的时间分别与视网膜顶盖和顶盖通径的时间几乎同步（Crossland等人，1975; Thanos和Bonhoeffer，1987; Wu等人，2000年）。这些研究结果，结合本研究，表明retinotectofugal系统成为建立，至少在结构水平上，胚胎天E8。
The avian retinotectofugal pathway has been suggested to be homologous to the mammalian retinotectofugal pathway. The projection of the nucleus rotundus upon the ectostriatum is equivalent to that of the pulvinar nucleus upon the extrastriate cortex in mammals. In this system, the optic tectum relays retinal input to the nucleus rotundus, which then ascends to the ectostriatum of the telencephalon. Given the fact that the chick retinotectofugal system becomes mature early during development, the present study attempted to investigate the timecourse of neuronal connections of the embryonic rotundoectostriatal pathway. We used multiple injections of cholera toxin B subunit (CTb) in the ectostriatum of chick embryos to retrogradely trace projections to the nucleus rotundus. We found CTb-labeled neurons in the nucleus rotundus at embryonic day 7.5–8. By embryonic day 8–8.5, increased numbers of CTb-labeled neurons were seen in the nucleus rotundus. It was noted that the time of this initial connection between the nucleus rotundus and the ectostriatum is nearly synchronous with that of the retinotectal and tectorotundal pathways, respectively (Crossland et al., 1975; Thanos & Bonhoeffer, 1987; Wu et al., 2000). These findings, combined with the present study, suggest that the retinotectofugal system becomes established, at least at a structural level, by embryonic day E8.