Neuroprotective Effect of Xueshuantong for Injection (Lyophilized) in Transient and Permanent Rat Cerebral Ischemia Model.
Neuroprotective Effect of Xueshuantong for Injection (Lyophilized) in Transient and Permanent Rat Cerebral Ischemia Model.
复制标题
Xueshuantong对瞬态和永久性大鼠脑缺血模型注射（冻干）的神经保护作用。
DOI：
10.1155/2015/134685
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Chen L
中科院分区：
文献类型：
--
作者：
Wang X;Wang S;Wang J;Guo H;Dong Z;Chai L;Hu L;Zhang Y;Wang H;Chen L
注射用血栓通（冻干）（XST）是从三七中提取的中药标准品，在我国临床上广泛用于急性脑梗死等脑血管疾病的治疗。本研究采用大鼠脑缺血模型，观察了XST的急性和持续保护作用，并探讨了XST对过氧化物氧还蛋白（Prx）6-Toll样受体（TLR）4信号通路的影响。结果表明，XST治疗3d可显著抑制大脑中动脉闭塞（MCAO）所致的脑梗死体积和肿胀率，并调节脑组织中白细胞介素1β（IL-1β）、IL-17、IL-23 p19、肿瘤坏死因子α（TNFα）和诱导型一氧化氮合酶（iNOS）的mRNA表达。进一步的研究表明，XST处理抑制过氧化物酶（Prx）6-toll样受体（TLR）4的蛋白表达和p38的磷酸化水平，上调STAT 3的磷酸化水平。XST可缩小永久性MCAO大鼠的梗死体积和肿胀度。此外，我们的研究结果显示，XST治疗可以增加大鼠的体重，并改善一系列功能结果。提示XST对短暂性和永久性MCAO大鼠脑缺血损伤具有保护作用，其机制可能与Prx 6-TLR 4通路有关。
Xueshuantong for Injection (Lyophilized) (XST), a Chinese Materia Medica standardized product extracted from Panax notoginseng (Burk.), is used extensively for the treatment of cerebrovascular diseases such as acutely cerebral infarction clinically in China. In the present study, we evaluated the acute and extended protective effects of XST in different rat cerebral ischemic model and explored its effect on peroxiredoxin (Prx) 6-toll-like receptor (TLR) 4 signaling pathway. We found that XST treatment for 3 days could significantly inhibit transient middle cerebral artery occlusion (MCAO) induced infarct volume and swelling percent and regulate the mRNA expression of interleukin-1β (IL-1β), IL-17, IL-23p19, tumor necrosis factor-α (TNFα), and inducible nitric oxide synthase (iNOS) in brain. Further study demonstrated that treatment with XST suppressed the protein expression of peroxiredoxin (Prx) 6-toll-like receptor (TLR) 4 and phosphorylation level of p38 and upregulated the phosphorylation level of STAT3. In permanent MCAO rats, XST could reduce the infarct volume and swelling percent. Moreover, our results revealed that XST treatment could increase the rats' weight and improve a batch of functional outcomes. In conclusion, the present data suggested that XST could protect against ischemia injury in transient and permanent MCAO rats, which might be related to Prx6-TLR4 pathway.